Qualitative and quantitative determination of ten alkaloids in traditional Chinese medicine Corydalis yanhusuo WT Wang by LC-MS/MS and LC-DAD
Qualitative and quantitative determination of ten alkaloids in traditional Chinese medicine Corydalis yanhusuo WT Wang by LC-MS/MS and LC-DAD
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DOI：
10.1016/j.jpba.2007.06.009
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发表时间：
2007-10-18
影响因子：
3.4
通讯作者：
Wu, Yutian
中科院分区：
文献类型：
--
作者：
Ding, Bo;Zhou, Tingting;Wu, Yutian
开发了一种具有MS和UV检测的高性能液相色谱（HPLC）的分析方法，用于用于对Corydalis Yanhusuo中生物碱的定性和定量确定。通过比较它们的保留时间，紫外和MS光谱与真实化合物的紫外线和MS光谱，可以鉴定出十种生物碱，包括七个三级生物碱和三个季季生物碱。此外，研究了[M+H]（+）和[M]（+）离子的碰撞诱导的解离，以阐明不同类型的生物碱的NIS行为。在阳性离子电喷雾电离串联串联（ESI-MS）中，所有三级生物碱均产生显着的[M+H]（+）离子，而Quaternary生物碱在一阶质谱中产生了突出的[M]（+）离子。在MS/MS光谱中观察到涉及H，CH3，CO，H2O和OCH3损失的片段。此外，通过这种方法对甲醇和乙酸乙酸乙酯提取物中的10个生物碱进行定量，通过这种方法进行了定量，该方法为评估Corydalis Yanhusuo制剂质量的新工具提供了新的工具。该方法为迄今为止Corydalis Yanhusuo中生物碱的表征和定量确定提供了最佳的敏感性和特异性。 （c）2007 Elsevier B.V.保留所有权利。
An analytical method incorporating high-performance liquid chromatography (HPLC) with MS and UV-detection was developed for the qualitative and quantitative determination of alkaloids in Corydalis yanhusuo. Ten alkaloids, including seven tertiary alkaloids and three quaternary alkaloids, were identified by comparing their retention times, UV and MS spectra with those of authentic compounds. Furthermore, the collision-induced dissociations of the [M + H](+) and [M](+) ions were studied to clarify the NIS behavior of the different types of alkaloids. In positive ion electrospray ionization tandem mass spectrometry (ESI-MS) all the tertiary alkaloids yielded prominent [M+H](+) ions and quaternary alkaloids yielded prominent [M](+) ions in the first order mass spectra. Fragments involving losses of H, CH3, CO, H2O and OCH3 were observed in the MS/MS spectra. In addition, quantification of the 10 alkaloids in Corydalis yonhusuo from methanol and ethyl acetate extract of different origins were performed by this method, which provides a new tool for the assessment of quality of Corydalis yanhusuo preparations. The method provides the best sensitivity and specificity for characterization and quantitative determination of the alkaloids in Corydalis yanhusuo so far. (c) 2007 Elsevier B.V. All rights reserved.