Intracisternal administration of chemokines facilitated formalin-induced behavioral responses in the orofacial area of freely moving rats
Intracisternal administration of chemokines facilitated formalin-induced behavioral responses in the orofacial area of freely moving rats
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DOI：
10.1016/j.brainresbull.2005.03.015
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发表时间：
2005-07-15
影响因子：
3.8
通讯作者：
Park, JS
中科院分区：
文献类型：
--
作者：
Ahn, DK;Lee, KR;Park, JS
本研究探讨了脑池内注射MCP-1、Rantes或IL-8对口面部疼痛传导的影响。我们还研究了脑池内注射IL-8产生的痛觉过敏反应的机制。采用口面福尔马林试验评估趋化因子对伤害性加工的影响。对于每只动物，连续9次5分钟记录行为反应的数量以及梳理、摩擦和/或抓挠福尔马林注射部位近端面部区域所花费的时间。脑池内给予MCP-1、Rantes或IL-8显著增加福尔马林诱导的口面区域的抓挠行为反应。脑池内用吲哚美辛（一种非选择性环氧合酶抑制剂）预处理不能阻断IL-8诱导的痛觉过敏。用100 μ g普萘洛尔（一种非选择性β-肾上腺素能受体拮抗剂）和50 μ g阿替洛尔（一种选择性β-肾上腺素能受体拮抗剂）预处理，可抑制脑池内注射1 ng IL-8产生的抓挠次数和抓挠持续时间。这些结果表明脑池内给予趋化因子在口面炎性疼痛模型中产生痛觉过敏反应，并且IL-8诱导的痛觉过敏由中枢β（1）-肾上腺素能受体介导。(c)2005年爱思唯尔公司All rights reserved.
The present study investigated the effects of intracistemal administration of MCP-1, Rantes or IL-8 on pain transmission in the orofacial area. We also investigated mechanisms of hyperalgesic responses produced by intracistemal administration of IL-8. An orofacial formalin test was employed to assess the effects of chemokines on nociceptive processing. For each animal, the number of behavioral responses and the time spent grooming, rubbing and/or scratching the facial region proximal to the formalin injection site was recorded for nine successive 5-min intervals. Intracisternal administration of MCP-1, Rantes or IL-8 significantly increased formalin-induced scratching behavioral responses in the orofacial area. Intracisternal pretreatment with indomethacin, a non-selective cyclooxygenase inhibitor, did not block IL-8-induced hyperalgesia. Pretreatment with 100 mu g propranolol, a non-selective beta-adrenergic receptor antagonist and 50 mu g atenolol, a selective beta-adrenergic receptor antagonist, inhibited the number of scratches and the duration of scratching produced by 1 ng of IL-8 injected intracistemally. These results indicate that intracisternal administration of chemokines produce a hyperalgesic response with an orofacial inflammatory pain model and that the IL-8-induced hyperalgesia is mediated by central beta(1)-adrenergic receptor. (c) 2005 Elsevier Inc. All rights reserved.