Tunable Thermoresponsiveness of Resilin via Coassembly with Rigid Biopolymers.
Tunable Thermoresponsiveness of Resilin via Coassembly with Rigid Biopolymers.
复制标题
通过与刚性生物聚合物共组装可调节 Resilin 的热响应性。
作者：
Jasmin L. Whittaker;N. Dutta;R. Knott;G. McPhee;N. Voelcker;C. Elvin;A. Hill;N. Choudhury
本研究通过一个简单的共组装系统来调节重组resilin蛋白Rec1-resilin的热响应性。研究了刚性模型多肽(多L-脯氨酸)或疏水刚性蛋白(家蚕丝素)对REC1-resilin在溶液中构象和形态随时间变化的影响以及REC1-resilin在溶液中的响应行为。观察到，通过诱导更多的有序构象和增加疏水性，体系的较低临界溶液温度(LCST)被调节到较低的值。时间和温度被发现是控制Rec1-resilin在模型多肽和更复杂的蛋白质系统中共组装行为的关键参数。这种独特的性质在广泛的应用中很有用，包括药物输送和软组织工程应用。
The ability to tune the thermoresponsiveness of recombinant resilin protein, Rec1-resilin, through a facile coassembly system was investigated in this study. The effects of change in conformation and morphology with time and the responsive behavior of Rec1-resilin in solution were studied in response to the addition of a rigid model polypeptide (poly-l-proline) or a hydrophobic rigid protein (Bombyx mori silk fibroin). It was observed that by inducing more ordered conformations and increasing the hydrophobicity the lower critical solution temperature (LCST) of the system was tuned to lower values. Time and temperature were found to be critical parameters in controlling the coassembly behavior of Rec1-resilin in both the model polypeptide and more complex protein systems. Such unique properties are useful for a wide range of applications, including drug delivery and soft tissue engineering applications.
登录
查看更多内容
基因工程丝 - 类似雌激素的蛋白质聚合物的可调自组装。
影响因子：
6.2
作者：
Xia XX;Xu Q;Hu X;Qin G;Kaplan DL
通讯作者：
Kaplan DL
影响因子：
3.3
作者：
Matsumoto, Akira;Chen, Jingsong;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
3.4
作者：
Greving, Imke;Dicko, Cedric;Vollrath, Fritz
通讯作者：
Vollrath, Fritz
影响因子：
6.2
作者：
Lu, Qiang;Zhu, Hesun;Zhang, Cencen;Zhang, Feng;Zhang, Bing;Kaplan, David L.
通讯作者：
Kaplan, David L.