EXTRACELLULAR POTENTIALS RELATED TO INTRACELLULAR ACTION POTENTIALS IN DOG PURKINJE SYSTEM
EXTRACELLULAR POTENTIALS RELATED TO INTRACELLULAR ACTION POTENTIALS IN DOG PURKINJE SYSTEM
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DOI：
10.1161/01.res.30.5.505
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发表时间：
1972-01-01
影响因子：
20.1
通讯作者：
JOHNSON, EA
中科院分区：
文献类型：
--
作者：
SPACH, MS;SERWER, GA;JOHNSON, EA
使用50µ钨细胞外电极和特殊保护细胞内微电极，对犬左心室浦肯野束的正常动作电位、膜部分去极化（通过早搏或细胞外钾升高）时启动的动作电位和低温下的动作电位进行细胞外和细胞内同步记录。细胞外波形的峰-峰幅度与细胞内动作电位的最大上升速率成正比，细胞外波形的持续时间与细胞内电位上升的持续时间成正比。细胞外电位的波形计算从记录的细胞内电位的方程，其中包括膜电流远离观察点的影响。在所有情况下，计算的波形准确地再现了记录的细胞外波形，并且波形不直接与细胞内电位的二阶空间导数或二阶时间导数成比例。细胞外电位被证明是直接相关的细胞内电位的空间分布，因此是一个敏感的传播指数和信息的来源，以前被认为是可获得的只有一个胞内电极的种类。
Simultaneous extracellular and intracellular recordings of normal action potentials, action potentials initiated at a time when the membrane was partially depolarized (by premature beats or elevated extracellular potassium), and action potentials at reduced temperature were made for Purkinje strands from the left ventricle of the dog with a 50µ tungsten extracellur electrode and a special guarded intracellular microelectrode. The peak-to-peak amplitude of the extracellular wave form was proportional to the maximum rate of rise of the intracellular action potential, and the duration of the extracellular wave form was proportional of the duration of the upstroke of the intracellular potential. Wave forms of extracellular potentials were computed from the recorded intracellular potentials with an equation which included the effects of membrane currents away from the point of observation. The computed wave forms accurately reproduced the recorded extracellular wave forms in all cases, and the wave forms were not directly porportional to the second spatial derivative or the second temporal derivative of the intracellular potential. Extracellular potentials are shown to be directly related to the spatial distribution of the intracellular potential and as such are a sensitive index of propagation and a source of information of the kind previously thought to be obtainable only with an intracllular electrode.