Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.
Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.
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脊尾虾抗脂多糖因子的多种异构体及其抗菌功能
DOI：
10.3390/md16050145
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发表时间：
2018-04-27
期刊：
影响因子：
5.4
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Lv X;Li S;Zhang C;Xiang J;Li F
As a kind of antimicrobial peptides (AMP) in crustacean, anti-lipopolysaccharide factors (ALFs) have broad spectrum antimicrobial activities. In the present study, we identified four ALF genes, EcALF2-5, from the ridgetail prawn Exopalaemon carinicauda. Tissue distribution analysis showed that EcALF2 and EcALF4 transcripts were mainly located in gill, epidermis, and stomach, while EcALF3 and EcALF5 were mainly in hemocytes. Peptides corresponding to the LPS binding domain (LBD) of EcALFs were synthesized for analyzing their antimicrobial activities. Minimal inhibitory concentration (MIC) analysis showed that the synthetic LBD peptides of EcALF3 and EcALF4 could inhibit the growth of Gram-positive and Gram-negative bacteria, while the synthetic LBD peptides of EcALF2 and EcALF5 showed antibacterial activity against Vibrio. Incubation of white spot syndrome virus (WSSV) with the synthetic LBD peptides of EcALF3, EcALF4, and EcALF5 could reduce the in vivo viral copy number in WSSV-infected prawns. After silencing of EcALFs, Vibrio exhibited a rapid proliferation in the hepatopancreas of the prawn. The present data showed the important function of different EcALFs in modulating the in vivo bacterial and viral propagation in E. carinicauda. This study will provide new clues into the disease control in aquaculture.
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DOI：
10.1186/1747-597x-9-11
发表时间：
2014-02-24
期刊：
Substance abuse treatment, prevention, and policy
影响因子：
--
作者：
Jiang H;Han Y;Du J;Wu F;Zhang R;Zhang H;Wang J;Zhou Z;Hser YI;Zhao M
通讯作者：
Zhao M
影响因子：
7.3
作者：
Martin L;van Meegern A;Doemming S;Schuerholz T
通讯作者：
Schuerholz T
影响因子：
3.6
作者：
Nagoshi, Hiroki;Inagawa, Hiroyuki;Soma, Gen-Ichiro
通讯作者：
Soma, Gen-Ichiro
影响因子：
5.4
作者：
Hancock, GE;Speelman, DJ;Cosco, C
通讯作者：
Cosco, C
虾抗脂多糖因子异构体的功能多样性及其与抗病毒活性相关的特征
影响因子：
5.4
作者：
Li S;Guo S;Li F;Xiang J
通讯作者：
Xiang J