Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.)
Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.)
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DOI：
10.1007/s00122-004-1785-7
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Forster, JW
中科院分区：
文献类型：
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作者：
Faville, MJ;Vecchies, AC;Forster, JW
利用分子标记构建了多年生黑麦草（Lolium perenne L.）已经通过使用与表达序列标签（EST）相关的多态性来构建。来自北非生态型和栽培品种Aurora的基因型之间的配对杂交用于产生双向假测交群体。选择了157个cDNA，分配到与农艺学上重要的生物学过程相关的8个不同功能类别，用于检测F-1（NA（6）x Au（6））群体中的多态性EST-RFLP位点。通过对14,767个单基因的分析，建立了一套完整的EST-SSR标记，其中310对引物表现出有效的扩增，检测到113个多态性位点。NA（6）遗传图谱包含88个EST-RFLP和71个EST-SSR位点，总长度为963 cM; Au（6）遗传图谱包含67个EST-RFLP和58个EST-SSR位点，总长度为757 cM。桥接基因座允许同源染色体之间的亲本地图的对齐，和一个子集的基因组DNA衍生的SSR被用来与连锁群的多年生黑麦草参考地图。分离扭曲的地区进行了鉴定，在某些情况下，在共同与其他多年生黑麦草地图。基于EST的标记图谱为计算机比较遗传作图以及QTL与功能相关遗传位点的共定位评价提供了基础。
A molecular marker-based map of perennial ryegrass ( Lolium perenne L.) has been constructed through the use of polymorphisms associated with expressed sequence tags ( ESTs). A pair-cross between genotypes from a North African ecotype and the cultivar Aurora was used to generate a two-way pseudo-testcross population. A selection of 157 cDNAs assigned to eight different functional categories associated with agronomically important biological processes was used to detect polymorphic EST - RFLP loci in the F-1( NA(6) x AU(6)) population. A comprehensive set of EST - SSR markers was developed from the analysis of 14,767 unigenes, with 310 primer pairs showing efficient amplification and detecting 113 polymorphic loci. Two parental genetic maps were produced: the NA(6) genetic map contains 88 EST - RFLP and 71 EST - SSR loci with a total map length of 963 cM, while the AU(6) genetic map contains 67 EST - RFLP and 58 EST - SSR loci with a total map length of 757 cM. Bridging loci permitted the alignment of homologous chromosomes between the parental maps, and a sub-set of genomic DNA-derived SSRs was used to relate linkage groups to the perennial ryegrass reference map. Regions of segregation distortion were identified, in some instances in common with other perennial ryegrass maps. The EST-derived marker-based map provides the basis for in silico comparative genetic mapping, as well as the evaluation of co-location between QTLs and functionally associated genetic loci.