Preparation of affinity membranes using polymer phase separation and azido-containing surfactants
Preparation of affinity membranes using polymer phase separation and azido-containing surfactants
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DOI：
10.1016/j.colsurfa.2020.125802
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发表时间：
2020-10
期刊：
影响因子：
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通讯作者：
K. Morita;Shinano Takeda;A. Yunoki;Takane Tsuchii;Tsutomu Tanaka;T. Maruyama
中科院分区：
文献类型：
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作者：
K. Morita;Shinano Takeda;A. Yunoki;Takane Tsuchii;Tsutomu Tanaka;T. Maruyama
我们提出了一种使用含叠氮基表面活性剂和点击化学来制备亲和膜的新方法。使用醋酸纤维素在含叠氮基表面活性剂存在下通过聚合物相分离制备多孔聚合物膜。热诱导相分离和非溶剂诱导相分离用于膜制备。通过 Huisgen 1,3-偶极环加成将膜表面上显示的叠氮基与次氮基三乙酸缀合，以制备对六组氨酸标记蛋白具有亲和力的膜。两种类型的相分离成功地生产了具有不同微观结构但表现出相似的分离性能的多孔膜。 d-Ala-d-Ala 代替次氮基三乙酸缀合至叠氮基官能化膜的表面。用 d-Ala-d-Ala 功能化的膜显示出对万古霉素的高亲和力。本方法使得聚合物材料易于表面功能化，并产生在其表面上展示各种类型配体的亲和膜。
We propose a novel approach to prepare affinity membranes using azido-containing surfactants and click chemistry. Porous polymeric membranes were prepared using cellulose acetate via polymer phase separation in the presence of azido-containing surfactants. Thermally induced phase separation and nonsolvent-induced phase separation were used for membrane preparation. The azido groups displayed on the membrane surfaces were conjugated with nitrilotriacetic acid via Huisgen 1,3-dipolar cycloaddition to prepare membranes that displayed affinity toward a hexahistidine-tagged protein. Two types of phase separation successfully produced porous membranes with different microstructures but showed similar separation performances. In place of nitrilotriacetic acid,d-Ala-d-Ala was conjugated to the surface of the azido-functionalized membrane. The membranes functionalized withd-Ala-d-Ala showed high affinity toward vancomycin. The present approach leads to facile surface functionalization of polymeric materials and produces affinity membranes displaying various types of ligands on their surfaces.