Polyethyleneimine modified fluorescent carbon dots and their application in cell labeling
Polyethyleneimine modified fluorescent carbon dots and their application in cell labeling
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DOI：
10.1016/j.colsurfb.2012.05.016
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发表时间：
2012-12-01
影响因子：
5.8
通讯作者：
Xu, Shukun
中科院分区：
文献类型：
--
作者：
Han, Baofu;Wang, Wenxing;Xu, Shukun
以葡萄糖为碳源，在水-乙二醇介质中溶剂热法合成了荧光碳点（CD），并首次采用聚乙烯亚胺（PEI）对其进行修饰，以提高其荧光性能。所制备的CD是单分散的球形颗粒，直径约为7.5 nm，发射出强烈的荧光，这是激发波长依赖性的，量子产率为3.5%。PEI修饰后，荧光强度增强了300倍，发射波长也发生了红移。然后将所制备的CD与CEA 8抗体缀合以在体外标记和成像HeLa细胞。我们还使用HeLa细胞测试了这些CD的细胞毒性。未观察到明显的细胞毒性，表明与CdTe量子点相比具有更好的生物安全性。(c)2012爱思唯尔有限公司版权所有。
Fluorescent carbon dots (CDs) were solvothermaly synthesized in water-glycol medium by using glucose as carbon source and then modified with polyethyleneimine (PEI) for the first time to improve fluorescence quality. The as-prepared CDs were monodispersed sphere particles with a diameter of about 7.5 nm, emitting strong fluorescence which is excitation wavelength-dependent, with a quantum yield of 3.5%. After PEI modification, there was a 300-fold enhancement in fluorescence intensity and also a red shift in emission wavelength. The as-prepared CDs were then conjugated with CEA8 antibody to label and image HeLa cells in vitro. We also tested the cytotoxicity of these CDs using HeLa cells. No apparent cytotoxicity was observed, demonstrating much better biosafety property compared with CdTe quantum dots. (c) 2012 Elsevier B.V. All rights reserved.