The ontogeny of fatty acid-binding protein in turkey (Meleagridis gallopavo) intestine and yolk sac membrane during embryonic and early posthatch development
The ontogeny of fatty acid-binding protein in turkey (Meleagridis gallopavo) intestine and yolk sac membrane during embryonic and early posthatch development
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DOI：
10.1093/ps/81.7.1065
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发表时间：
2002-07-01
期刊：
影响因子：
4.4
通讯作者：
Lilburn, MS
中科院分区：
文献类型：
--
作者：
Ding, ST;Lilburn, MS
本试验旨在研究火鸡幼雏胚胎发育及孵化后早期肠道和卵黄囊膜中脂肪酸结合蛋白（FABP）的存在和个体发生。肠（I-）FABP的测量使用免疫印迹程序纳入抗鸡肝（L-）FABP抗血清。FABP活性在两个组织中也证实了配体结合试验纳入C-14-油酸。I-FABP与鸡L-FABP抗血清直到孵化，胚胎第28天（艾德28）才发生交叉反应，此后I-FABP浓度在孵化后第一个3天（PD 3）增加了39%。然而，在总肠基础上计算的FABP浓度（ng/肠）在PD 6时增加了10倍。比活性[每分钟崩解数（dpm）/mg胞质蛋白]在孵化时最大，此后略有下降，而卵黄囊膜中FABP的比活性在艾德16和艾德19之间达到峰值，然后下降。总卵黄囊活性（dpm/卵黄囊膜），然而，平台艾德在艾德22下降到低水平之前，PD 3，与最大的脂质转移出蛋黄的时期相一致。
Experiments were conducted to confirm the existence and ontogeny of fatty acid binding protein (FABP) in the intestine and yolk sac membrane of turkey poults (Meleagridis gallopavo) during embryonic and early posthatch development. Intestinal (I-) FABP was measured using an immunoblot procedure incorporating anti-chick liver (L-)FABP antisera. FABP activity in both tissues was also confirmed with a ligand-binding assay incorporating C-14-oleic acid. I-FABP did not cross-react with chick L-FABP antisera until hatch, embryonic day 28 (ED 28), after which there was a 39% increase in I- FABP concentration through the first 3 d posthatch (PD 3). FABP concentration calculated on a total intestinal basis (ng/intestine), however, increased 10-fold through PD 6. Specific activity [disintegrations per minute (dpm)/mg cytosolic protein] was greatest at hatch and decreased slightly thereafter, whereas specific activity of FABP in the yolk sac membrane peaked between ED 16 and ED 19 and then declined. Total yolk sac activity (dpm/yolk sac membrane), however, plateaued at ED 22 before declining to low levels by PD 3, coincident with the period of maximal lipid transfer out of the yolk.