Detection and characterization of IS256, an insertion sequence in Staphylococcus aureus.
Detection and characterization of IS256, an insertion sequence in Staphylococcus aureus.
复制标题
金黄色葡萄球菌中的插入序列 IS256 的检测和表征。
DOI：
10.1099/00221287-133-11-3031
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Skurray
中科院分区：
文献类型：
--
作者：
B. Lyon;M. Gillespie;R. Skurray
澳大利亚分离的金黄色葡萄球菌对氨基糖苷类庆大霉素(GMR)、妥布霉素(TMR)和卡那霉素(KMR)的耐药性是由转座子Tn4001介导的。该转座子的1.35kb反向重复序列表现出插入序列的许多特征，因此被命名为IS256。IS256与Tn4001的串联复制导致GmrTmrKmr水平增加，从而意味着该元件具有强大的启动子序列。已经观察到IS256在葡萄球菌染色体上的连续插入和独立插入，后者提示该元件可能在基因组的分子重排中发挥作用。
Resistance to the aminoglycosides gentamicin (Gmr), tobramycin (Tmr) and kanamycin (Kmr) in strains of Staphylococcus aureus isolated in Australia is mediated by the transposon Tn4001. The 1.35 kb inverted repeat of this transposon exhibits many of the characteristics of an insertion sequence and has consequently been designated IS256. Tandem duplication of IS256 contiguous with Tn4001 results in an increase in the level of GmrTmrKmr, thereby implying that the element possesses strong promoter sequences. Both contiguous and independent insertions of IS256 into the staphylococcal chromosome have been observed, the latter suggesting that the element may play a role in molecular rearrangements of the genome.