Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor
Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor
复制标题
DOI：
10.1007/s00253-005-0046-2
复制
发表时间：
2006-03-01
影响因子：
5
通讯作者：
Tsuneda, S
中科院分区：
文献类型：
--
作者：
Isaka, K;Date, Y;Tsuneda, S
测定了厌氧生物过滤（ABF）反应器中厌氧氨氧化菌的倍增时间。采用荧光原位杂交法对厌氧污泥中的厌氧菌细胞进行检测和计数。结果表明，第14和21 d厌氧氨氧化菌的数量分别为1.1x10(6)和1.7x10(7)个细胞/ml反应器。由此计算出厌氧氨氧化菌的倍增时间为1.8 d，比生长率（mu）为0.39 d（-1）。该结果表明厌氧氨氧化菌的生长速度高于报道值（翻倍时间，11天）。此外，氮的转化率与厌氧氨氧化菌的数量成正比。在ABF反应器中保持厌氧氨氧化菌生长的理想环境可能会导致更快的生长。这是基于厌氧氨氧化菌直接计数的厌氧氨氧化菌生长速率的首次报道。
The doubling time of anaerobic ammonium-oxidizing (anammox) bacteria in an anaerobic biological filtrated (ABF) reactor was determined. Fluorescence in situ hybridization analysis was used to detect and count anammox bacteria cells in anammox sludge. As a result, the populations of anammox bacteria at 14th and 21st days were 1.1x10(6) and 1.7x10(7) cells/ml reactor, respectively. From these results, the doubling time of anammox bacteria was calculated as 1.8 days, and the specific growth rate (mu) was 0.39 day(-1). This result indicated that the anammox bacteria have higher growth rate than the reported value (doubling time, 11 days). Furthermore, it was clearly demonstrated that nitrogen conversion rate was proportional to the population of anammox bacteria. Maintaining the ideal environment for the growth of anammox bacteria in the ABF reactor might lead to faster growth. This is the first report of the growth rate of anammox bacteria based on the direct counting of anammox bacteria.