In Vitro Evaluation of the Effect of Stimulation with Magnetic Fields on Chondrocytes.
In Vitro Evaluation of the Effect of Stimulation with Magnetic Fields on Chondrocytes.
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DOI：
10.1002/bem.22231
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发表时间：
2020-01-01
影响因子：
1.9
通讯作者：
Garzon-Alvarado, Diego Alexander
中科院分区：
文献类型：
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作者：
Escobar, Juan Felipe;Vaca-Gonzalez, Juan Jairo;Garzon-Alvarado, Diego Alexander
磁场（MF）已被用作外部刺激，以增加软骨细胞的细胞增殖和关节软骨的细胞外基质（ECM）合成。然而，以前发表的研究没有表明，MF是均匀的通过细胞培养系统。此外，诸如刺激时间和MF强度的变量尚未标准化以获得最佳细胞增殖速率或ECM分子合成的增加。在这项工作中，刺激装置，产生均匀的MF刺激细胞培养表面的设计和制造使用的计算模型。此外，建立了原代大鼠软骨细胞的体外培养物，并用两种MF方案刺激以测量增殖和ECM合成。以2 mT的磁场作用3 h，每6 h一次，共8d，增殖率最高。此外，当用2 mT的MF刺激细胞5小时，每6小时一次，持续8天时，糖胺聚糖的合成增加具有统计学显著性。这些发现表明，用MFs刺激是一种有前途的工具，可用于改善体外治疗，如自体软骨细胞植入，以增加细胞增殖或刺激分子合成。生物电磁学2020;41：41-51 © 2019生物电磁学学会。
Magnetic fields (MFs) have been used as an external stimulus to increase cell proliferation in chondrocytes and extracellular matrix (ECM) synthesis of articular cartilage. However, previously published studies have not shown that MFs are homogeneous through cell culture systems. In addition, variables such as stimulation times and MF intensities have not been standardized to obtain the best cellular proliferative rate or an increase in molecular synthesis of ECM. In this work, a stimulation device, which produces homogeneous MFs to stimulate cell culture surfaces was designed and manufactured using a computational model. Furthermore, an in vitro culture of primary rat chondrocytes was established and stimulated with two MF schemes to measure both proliferation and ECM synthesis. The best proliferation rate was obtained with an MF of 2mT applied for 3h, every 6h for 8 days. In addition, the increase in the synthesis of glycosaminoglycans was statistically significant when cells were stimulated with an MF of 2mT applied for 5h, every 6h for 8 days. These findings suggest that a stimulation with MFs is a promising tool that could be used to improve in vitro treatments such as autologous chondrocyte implantation, either to increase cell proliferation or stimulate molecular synthesis. Bioelectromagnetics. 2020;41:41-51 © 2019 Bioelectromagnetics Society.