Role of differential mRNA stability in the regulated expression of IgM and IgD.
Role of differential mRNA stability in the regulated expression of IgM and IgD.
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差异 mRNA 稳定性在 IgM 和 IgD 表达调节中的作用。
DOI：
10.4049/jimmunol.152.11.5330
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发表时间：
1994
影响因子：
4.4
通讯作者：
D. Yuan
中科院分区：
文献类型：
--
作者：
D. Reed;J. Hawley;T. Dang;D. Yuan
静息与活化B细胞中IgM和IgD重链mRNA的表达为评估翻译对mRNA稳定性的影响提供了一种独特的工具，因为mu和delta mRNA具有相同的VDJ序列，但在分子的其余部分存在很大差异。我们已经证明，尽管5' UTR的身份允许平等地进入翻译机制，但mu mRNA的周转率明显高于delta mRNA。而mu mRNA短t1/2在B细胞活化后显著增加。此外，B细胞活化后microS mRNA的诱导为比较提供了另一种相关分子。因此，尽管microS和microM mRNA的3'端不同，但它们在活化的B细胞中具有相同的周转率。此外，由于δ mRNA、β 2和GAPDH mRNA的周转率保持不变，这些实验表明，B细胞活化导致了靶向mRNA-mu中特定序列的调节因子的诱导，从而赋予了更大的稳定性。他们还反对由于分泌机制的改变而对mRNA稳定性进行更被动的调节。
The expression of IgM and IgD heavy chain mRNA in resting vs activated B cells offers a unique tool for the assessment of the effect of translation on mRNA stability because mu and delta mRNA have identical VDJ sequences but differ substantially in the rest of the molecule. We have shown that despite the 5' UTR identity that allows equal access to the translation machinery, mu mRNA has a significantly higher turnover rate than delta mRNA. However, the short t1/2 of mu mRNA increases significantly after B cell activation. Furthermore, the induction of microS mRNA after B cell activation provides yet another related molecule for comparison. Thus, despite the fact that microS and microM mRNA differ at their 3' ends, they have identical turnover rates in activated B cells. In addition, because the turnover rates of delta mRNA and beta 2 and GAPDH mRNA remain unchanged, these experiments suggest that B cell activation results in the induction of regulatory factor(s) that target specific sequences within mRNA-mu to confer greater stability. They also argue against a more passive regulation of mRNA stability that is a consequence of alterations in the secretory machinery.