Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections
Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections
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DOI：
10.1111/j.1365-2591.2005.01053.x
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发表时间：
2006-01-01
影响因子：
5
通讯作者：
Souza, FJ
中科院分区：
文献类型：
--
作者：
Jacinto, RC;Gomes, BPFA;Souza, FJ
目的了解牙龈卟啉单胞菌在根尖周脓肿患者根管中的分布情况及对常用抗生素的敏感性。采用适当的厌氧菌种培养方法完成微生物采样、分离和细菌鉴定。采用E-test法对20株牙龈卟啉单胞菌进行药敏试验。结果共分离出352株细菌，分属于69个不同的种。严格厌氧菌占83%，革兰阴性菌占47.5%。牙龈卟啉单胞菌被发现在20根管，最常见的症状病例。牙龈卟啉单胞菌的存在与脓性渗出物和触诊疼痛有关（均P < 0.05）。所有牙龈卟啉单胞菌对阿莫西林、阿莫西林+克拉维酸、头孢克洛、克林霉素、苄青霉素、甲硝唑和四环素敏感。观察到对阿莫西林+克拉维霉素和克林霉素的最低抑菌浓度（MIC）（0.026-0.125 μ g mL（-1））的最低范围。观察到对克林霉素的最低MIC 90（0.064 μ g mL-1）。1株对红霉素耐药，8株对阿奇霉素耐药。结论牙龈卟啉单胞菌病原体在感染牙尖周脓肿的根管内频繁分离。阿莫西林、阿莫西林-克拉维酸和苄青霉素对牙龈卟啉单胞菌有效。
Aim To investigate the prevalence of Porphyromonas gingivalis in root canals of infected teeth with periapical abscesses and to investigate the antimicrobial susceptibility of this species to some frequently prescribed antibiotics.Methodology Samples were obtained from 70 root canals of abscessed teeth. Microbial sampling, isolation and bacterial identification were accomplished using appropriate culture methods for anaerobic species. The antimicrobial susceptibility of the 20 strains of P. gingivalis isolated was determined by using the E-test. The antimicrobial agents tested were amoxicillin, amoxicillin + clavulanate, azythromycin, benzylpenicillin, cephaclor, clindamycin, erythromycin, metronidazole and tetracycline.Results A total of 352 individual strains, belonging to 69 different species, were isolated. Eighty three percent of the strains were strict anaerobes and 47.5% of the isolated bacteria were Gram-negative. Porphyromonas gingivalis was found in 20 root canals and was most frequently found in symptomatic cases. Statistically, the presence of P. gingivalis was related to purulent exudates and pain on palpation (both P < 0.05). All P. gingivalis strains were sensitive to amoxicillin, amoxicillin + clavulanate, cephaclor, clindamycin, benzylpenicyllin, metronidazole and tetracycline. The lowest range of minimum inhibitory concentration (MIC) (0.026-0.125 mu g mL(-1)) was observed against amoxicillin + clavulanate and clindamycin. The lowest MIC 90 was observed against clindamycin (0.064 mu g mL(-1)). One strain was resistant to erythromycin and eight strains were resistant to azythromycin.Conclusion Porphyromonas gingivalis pathogen is isolated with frequency from root canals of infected teeth with periapical abscesses. Amoxicillin, as well as amoxicillin-clavulanic acid and benzylpenicillin were effective against P. gingivalis.