Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato
Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato
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DOI：
10.1038/ng2036
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发表时间：
2007-06-01
期刊：
影响因子：
30.8
通讯作者：
Eshed, Yuval
中科院分区：
文献类型：
--
作者：
Ori, Naomi;Cohen, Aya Refael;Eshed, Yuval
植物叶片在大小和形状上具有明显的可塑性。在经典的部分显性突变Lanceolate（La）1中，番茄（Solanum lycopersicum）的大复叶转化为小单叶。我们发现LA编码来自TCP家族的转录因子（2，3），其含有miR319结合位点（4）。五个独立的La分离株是由miR319结合位点内的点突变引起的获得性功能等位基因，并赋予La转录物对microRNA（miRNA）定向抑制的部分抗性。对miRNA调节的敏感性降低导致非常年轻的La叶原基中LA表达升高，并导致叶缘的早熟分化。相反，使用miR319的异位表达下调几个LA样基因导致更大的小叶和叶缘的连续生长。我们的研究结果表明，LA的miR 319的调节定义了一个灵活的窗口，形态发生能力沿着发展叶缘，叶加工所需的。
Plant leaves show pronounced plasticity of size and form. In the classical, partially dominant mutation Lanceolate ( La) 1, the large compound leaves of tomato (Solanum lycopersicum) are converted into small simple ones. We show that LA encodes a transcription factor from the TCP family(2,3) containing an miR319-binding site(4). Five independent La isolates are gain-offunction alleles that result from point mutations within the miR319-binding site and confer partial resistance of the La transcripts to microRNA (miRNA)-directed inhibition. The reduced sensitivity to miRNA regulation leads to elevated LA expression in very young La leaf primordia and to precocious differentiation of leaf margins. In contrast, downregulation of several LA-like genes using ectopic expression of miR319 resulted in larger leaflets and continuous growth of leaf margins. Our results imply that regulation of LA by miR319 defines a flexible window of morphogenetic competence along the developing leaf margin that is required for leaf elaboration.