Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniae
Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniae
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DOI：
10.1086/376571
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发表时间：
2003-08-01
影响因子：
6.4
通讯作者：
Benjamin, WH
中科院分区：
文献类型：
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作者：
Briles, DE;Hollingshead, SK;Benjamin, WH
在没有菌血症的情况下，某些荚膜组19肺炎链球菌菌株在小鼠鼻内感染可导致局灶性肺炎。利用这种小鼠肺炎模型，我们证明了重组肺炎球菌表面蛋白A （PspA）或PdB（溶肺素的一种遗传解毒衍生物）的免疫接种可引起对局灶性肺部感染的显著保护。这可能是首次证明拟议的疫苗抗原可以预防肺炎球菌性肺炎。PspA和PdB混合免疫小鼠获得的保护效果最好，说明这两种抗原所激发的保护作用是互补的。这一结果与先前使用肺炎球菌败血症和鼻腔定植模型的研究一致，并表明预防感染的最佳蛋白疫苗可能是那些包含多种引起保护的肺炎球菌蛋白的疫苗。
Intranasal infection of mice with certain strains of capsular group 19 Streptococcus pneumoniae can result in focal pneumonia in the absence of bacteremia. Using this model of murine pneumonia, we demonstrated that immunization with recombinant forms of either pneumococcal surface protein A ( PspA) or PdB ( a genetically detoxified derivative of pneumolysin) elicited significant protection against focal pulmonary infection. This may be the first demonstration that a proposed vaccine antigen can protect against pneumococcal pneumonia. The best protection was obtained by immunizing mice with a mixture of PspA and PdB, indicating that the protection elicited by these antigens can complement each other. This result is in agreement with previous studies that used pneumococcal sepsis and nasal colonization models and demonstrate that the best protein vaccines for prevention of infection may be those that include more than one protection-eliciting pneumococcal protein.