RID1, encoding a Cys2/His2-type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice
RID1, encoding a Cys2/His2-type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice
复制标题
DOI：
10.1073/pnas.0806019105
复制
发表时间：
2008-09-02
影响因子：
11.1
通讯作者：
Zhang, Qifa
中科院分区：
文献类型：
--
作者：
Wu, Changyin;You, Changjun;Zhang, Qifa
从营养生长阶段向生殖生长阶段的转变是高等植物生活史中的一个重要过程。虽然开花调控的分子机制已被广泛的特点，在一些植物物种，很少有人知道有关过渡过程如何启动。在这里，我们表明，水稻不确定1（RID 1）基因作为从营养阶段到生殖阶段的过渡的主开关。RID 1编码一个Cys-2/His-2型锌指转录因子，在拟南芥中没有直系同源物。通过T-DNA插入突变的RID 1敲除（rid 1）在一系列生长条件下生长>500天后从不抽穗，因此被称为从不开花表型。这种基因的突变抑制表达已知参与开花调节，特别是在Ehd 1/Hd 3a途径和一系列RFT同源物中。RID 1似乎与生物钟无关。提出了一个将RID 1置于水稻开花调控分子途径中的模型，其中有两个不可或缺的元件。首先，RID 1控制花诱导的相变和起始。在另一种情况下，Hd 3a/RFL 1/FTL复合物作为开花的直接诱导物。任何一个元素的功能丧失都会导致永不开花。一旦随着RID 1的激活而诱导相变，开花信号就会通过各种途径进行转导和调节，并最终与FT样蛋白整合以诱导开花。
Transition from the vegetative phase to reproductive phase is a crucial process in the life cycle of higher plants. Although the molecular mechanisms of flowering regulation have been extensively characterized in a number of plant species, little is known regarding how the transition process initiates. Here, we show that the Rice Indeterminate 1 (RID 1) gene acts as the master switch for the transition from the vegetative to reproductive phase. RID1 encodes a Cys-2/His-2-type zinc finger transcription factor that does not have an ortholog in Arabidopsis spp. A RID1 knockout(rid1), mutated by T-DNA insertion, never headed after growing for >500 days under a range of growth conditions and is thus referred to as a never-flowering phenotype. This mutation-suppressed expression of the genes is known to be involved in flowering regulation, especially in the Ehd1/Hd3a pathway and a series of RFT homologs. RID1 seems to be independent of the circadian clock. A model was proposed to place RID1 in the molecular pathways of flowering regulation in rice, for which there are two indispensable elements. In the first, RID1 is controlling the phase transition and initiation of floral induction. In the other, the Hd3a/RFL1/FTL complex acts as the immediate inducer of flowering. Loss of function in either element would cause never-flowering. Once the phase transition is induced with the activation of RID1, flowering signal is transduced and regulated through the various pathways and eventually integrated with FT-like proteins to induce flowering.