Improving longitudinal resolution of Airy beams two-photon volume imaging with fluorescence lifetime imaging
Improving longitudinal resolution of Airy beams two-photon volume imaging with fluorescence lifetime imaging
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DOI：
10.1016/j.optcom.2022.129151
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发表时间：
2022-11
影响因子：
2.4
通讯作者：
Yong Guo;Luwei Wang;Yinru Zhu;Xinwei Gao;Xiaoyu Weng;Jinyuan Liu;Wei Yan;J. Qu
中科院分区：
文献类型：
--
作者：
Yong Guo;Luwei Wang;Yinru Zhu;Xinwei Gao;Xiaoyu Weng;Jinyuan Liu;Wei Yan;J. Qu
已经报道了无衍射艾里光束通过将轴向显著伸长的信息投影到二维（2D）图像来提高采集速度。由于延长的焦距，艾里体积图像可以覆盖与在不同成像深度处具有多个高斯图像的堆叠图像一样多的信息，这显著地增加了体积成像速率。然而，三维（3D）结构在2D平面上的投影通常是不可区分的，尤其是那些沿轴沿着重叠的结构。因此，我们提出了一种基于双光子荧光寿命显微成像（FLIM）的轴向分辨体积成像技术。
The non-diffracting Airy beams have been reported to increase acquisition speed by projecting the axially significantly elongated information to a two-dimensional (2D) image. Due to the elongated focal length, an Airy volumetric image can cover information as much as stack images with multiple Gaussian images at different imaging depths, which significantly increases the volumetric imaging rate. However, the projection of three-dimensional (3D) structures on a 2D plane is usually indistinguishable, especially those that overlap along the axis. Therefore, we proposed an axially resolved volume imaging technique based on a two-photon fluorescence lifetime microscopy imaging (FLIM) by using Airy beams.