Incidence of Prevotella intermedia and Prevotella nigrescens in periodontal health and disease
Incidence of Prevotella intermedia and Prevotella nigrescens in periodontal health and disease
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DOI：
10.1111/j.1348-0421.1998.tb02328.x
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发表时间：
1998-01-01
影响因子：
2.6
通讯作者：
Fujita, H
中科院分区：
文献类型：
--
作者：
Maeda, N;Okamoto, M;Fujita, H
研究了牙周健康和牙周疾病中黑色素棒（BPRs），特别是中普雷沃氏菌和黑变普雷沃氏菌的发病率。此外，还比较了牙周健康菌株和牙周疾病菌株间媒介假单胞菌的降解酶活性。采用纸点法在龈下间隙或牙周袋处采集微生物标本。牙周健康者中有71.1% （n=45）存在BPRs，牙周疾病患者中有47.1% （n=34）和87.8% （n=41）存在BPRs。牙龈卟啉单胞菌仅在牙周病患者的活动部位检出（180株中占17.8%）。健康部位和活动部位的BPR均以中介体为主（分别为37.3%和41.7%）。在牙周健康人群中，黑黑假单胞菌是主要的BPR（占173株的70.5%）。患者体内活性部位分离的中间假单胞菌酯酶、酯酶-脂肪酶、酸性磷酸酶和聚焦酶活性显著高于健康人群（P< 0.05）。结果表明，在一定条件下，中间芽孢杆菌可能提高牙周炎降解酶的活性，支持牙周炎的进展。
The incidence of black-pigmented rods (BPRs), especially Prevotella intermedia and Prevotella nigrescens, in periodontal health and disease were examined. Furthermore, the degradative enzyme activities of P. intermedia were compared among the strains from periodontal health and disease. Microbiological specimens were collected from subgingival crevice or periodontal pocket by paper point. The BPRs were found in 71.1% of periodontally healthy subjects (n=45), and in 47.1% of healthy sites (n=34) and 87.8% of active sites (n=41) among periodontally diseased patients. Porphyromonas gingivalis was detected only in active sites of periodontally diseased patients (17.8% of 180 strains). Tt intermedia was the predominant BPR in both healthy and active sites (37.3 and 41.7%, respectively) of the patients. However, P. nigrescens was the predominant BPR (70.5% of 173 strains) in periodontally healthy subjects. The enzyme activities of esterase, esterase-lipase, acid-phosphatase and or-fucosidase of P. intermedia strains isolated from active sites in patients were significantly higher (P< 0.05) than those of healthy subjects. The results suggest that P. intermedia might increase the activity of degradative enzymes under a certain condition and support the progression of periodontitis.