Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division
Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division
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DOI：
10.1242/dev.087338
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发表时间：
2013-07-01
期刊：
影响因子：
4.6
通讯作者：
Bellaiche, Yohanns
中科院分区：
文献类型：
--
作者：
Jauffred, Bertrand;Llense, Flora;Bellaiche, Yohanns
不对称的细胞分裂在发育和成年期产生细胞命运的多样性。最近的研究表明，在干细胞分裂过程中，中心体的运动在前期是不对称的，这种不对称性参与了有丝分裂纺锤体的取向和细胞极化。在这里，我们研究了果蝇感觉器官前体不对称分裂过程中中心体的动态变化，发现在胞质分裂过程中中心体的运动是不对称的。我们证明，中心体的运动是由细胞命运决定簇Numb，它不通过其经典的效应器，Sanpodo和α-适应，但通过塌陷蛋白反应介导蛋白（CRMP）的作用。此外，我们发现，CRMP是必要的有效的Notch信号，它调节的Rab 11阳性内体，通过Notch配体，三角洲是回收的pericentriolar积累的持续时间。我们的工作特征的不对称分裂过程中的不对称中心体运动的一个额外的模式，并提出了一个模型，使中心体运动的不对称性参与差异Notch激活，以调节细胞命运的规范。
Asymmetric cell division generates cell fate diversity during development and adult life. Recent findings have demonstrated that during stem cell divisions, the movement of centrosomes is asymmetric in prophase and that such asymmetry participates in mitotic spindle orientation and cell polarization. Here, we have investigated the dynamics of centrosomes during Drosophila sensory organ precursor asymmetric divisions and find that centrosome movements are asymmetric during cytokinesis. We demonstrate that centrosome movements are controlled by the cell fate determinant Numb, which does not act via its classical effectors, Sanpodo and alpha-Adaptin, but via the Collapsin Response Mediator Protein (CRMP). Furthermore, we find that CRMP is necessary for efficient Notch signalling and that it regulates the duration of the pericentriolar accumulation of Rab11-positive endosomes, through which the Notch ligand, Delta is recycled. Our work characterizes an additional mode of asymmetric centrosome movement during asymmetric divisions and suggests a model whereby the asymmetry in centrosome movements participates in differential Notch activation to regulate cell fate specification.