ASSOCIATION OF INTEGRASE, MATRIX, AND REVERSE-TRANSCRIPTASE ANTIGENS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 WITH VIRAL NUCLEIC-ACIDS FOLLOWING ACUTE INFECTION
ASSOCIATION OF INTEGRASE, MATRIX, AND REVERSE-TRANSCRIPTASE ANTIGENS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 WITH VIRAL NUCLEIC-ACIDS FOLLOWING ACUTE INFECTION
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DOI：
10.1073/pnas.90.13.6125
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发表时间：
1993-07-01
影响因子：
11.1
通讯作者：
STEVENSON, M
中科院分区：
文献类型：
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作者：
BUKRINSKY, MI;SHAROVA, N;STEVENSON, M
我们已经研究了人类免疫缺陷病毒1型（HIV-1）的整合前复合物的组成部分，并分析了这些组成部分之间的联系。HIV-1核蛋白复合物，从急性病毒感染后的CD 4+细胞的细胞核和细胞质提取物中分离，含有与病毒基质（MA），整合酶（IN），逆转录酶（RT）抗原，但不衣壳（CA）抗原的病毒RNA和DNA，并具有整合活性在体外。与病毒DNA的IN抗原而非RT或MA抗原的结合是洗涤剂稳定的。病毒DNA合成和病毒核蛋白复合物的核输入的可逆RT抑制剂的存在下的分析表明，病毒RNA的逆转录可以完全在宿主细胞核中完成。我们的研究表明HIV-1的核蛋白（整合前）复合物的结构和功能特征，这与理解慢病毒生命周期中的早期事件有关。
We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral RNA and DNA in association with viral matrix (MA), integrase (IN), and reverse transcriptase (RT) antigens but not capsid (CA) antigens and possessed integration activity in vitro. Association of IN but not RT or MA antigens with viral DNA was detergent-stable. Analysis of viral DNA synthesis and nuclear import of viral nucleoprotein complexes in the presence of a reversible RT inhibitor demonstrated that reverse transcription of viral RNA could be completed entirely in the host cell nucleus. Our studies demonstrate structural and functional features of the nucleoprotein (preintegration) complex of HIV-1 which are pertinent to the understanding of early events in the lentiviral life cycle.