Direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry
Direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry
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DOI：
10.1016/j.jasms.2009.08.023
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发表时间：
2009-12
影响因子：
3.2
通讯作者：
Zhan Yu;L. Chen;Hiroaki Suzuki;O. Ariyada;R. Erra-Balsells;H. Nonami;K. Hiraoka
中科院分区：
文献类型：
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作者：
Zhan Yu;L. Chen;Hiroaki Suzuki;O. Ariyada;R. Erra-Balsells;H. Nonami;K. Hiraoka
探针电喷雾离子化（PESI）是一种新发展起来的电喷雾离子化技术。在这项研究中，我们已经应用PESI接口的飞行时间质谱仪（TOF-MS）的植物化学物质在一个部分的郁金香鳞茎在不同的区域，包括基板，鳞片的内外边缘，花蕾和树叶的直接配置。郁金香花瓣和叶片的不同部位也进行了研究。碳水化合物，氨基酸和其他植物化学物质的检测。采用活体PESI-MS技术，对郁金香鳞茎生长初期第一周的碳水化合物含量变化进行了研究。观察到碳水化合物的分解，这与通过其他技术实现的先前报告一致。本研究表明，PESI-MS具有样品消耗量少、样品制备量少等优点，可用于活体生物系统中植物化学物质的快速、直接分析。因此，PESI-MS可以成为直接分析/分析生物活性化合物或监测活生物系统中代谢变化的新选择。
Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems.