Optimized Surface-enhanced Raman Scattering (SERS) Colloids for the Characterization of Microorganisms
Optimized Surface-enhanced Raman Scattering (SERS) Colloids for the Characterization of Microorganisms
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DOI：
10.2116/analsci.26.761
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发表时间：
2010-07-01
影响因子：
1.6
通讯作者：
Haisch, Christoph
中科院分区：
文献类型：
--
作者：
Knauer, Maria;Ivleva, Natalia P.;Haisch, Christoph
我们报告了基于表面增强拉曼散射（SERS）的微生物原位分析的银胶体制备方法的比较。对不同的胶体溶胶作为 SERS 底物的适用性进行了测试和优化。通过 Leopold 和 Lendl 的修改程序产生的银溶胶对测试分子结晶紫给出了 10(8) 量级的拉曼信号增强因子。此外，该SERS底物还成功应用于免疫分析中固定微生物的原位检测和鉴定。胶体制备在室温下进行，胶体可稳定数周。这种用于微生物无标记原位检测的银纳米粒子制备方法成功地将SERS与免疫分析结合起来。因此，它在生物分子的高通量检测方面具有巨大的潜力。
We report on a comparison of silver colloid preparation methods for the in situ analysis of microorganisms based on surface-enhanced Raman scattering (SERS). Different colloid sols were tested and optimized regarding their suitability as SERS substrates. The silver sols produced by a modified procedure of Leopold and Lendl gave an enhancement factor of the Raman signal in the order 10(8) for the test molecule, crystal violet. Furthermore, this SERS substrate was successfully applied for the in situ detection and identification of microorganisms immobilized on an immunoassay. The colloid preparation was carried out at room temperature and the colloids were stable for weeks. This silver nanoparticle preparation method for the label-free in situ detection of microorganisms successfully combines SERS with immunoassays. Hence, it has a great potential for the high-throughput detection of biomolecules.