Knockout of rapC Improves the acillomycin D Yield Based on De Novo Genome Sequencing of Bacillus amyloliquefaciens fmbJ
Knockout of rapC Improves the acillomycin D Yield Based on De Novo Genome Sequencing of Bacillus amyloliquefaciens fmbJ
复制标题
基于解淀粉芽孢杆菌 fmbJ 的从头基因组测序，敲除 rapC 可提高阿西霉素 D 的产量
DOI：
10.1021/acs.jafc.8b00418
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lu Zhaoxin
中科院分区：
文献类型：
--
作者：
Sun Jing;Lu Zhaoxin
Bacillus amyloliquefaciens, a Gram-positive and soil-dwelling bacterium, could produce secondary metabolites that suppress plant pathogens. In this study, we provided the whole genome sequence results ofB. amyloliquefaciensfmbJ, which had one circular chromosome of 4 193 344 bp with 4249 genes, 87 tRNA genes, and 27 rRNA genes. In addition, fmbJ was found to contain several gene clusters of antimicrobial lipopeptides (bacillomycin D, surfactin, and fengycin), and bacillomycin D homologues were further comprehensively identified. To clarify the influence ofrapCregulating the synthesis of lipopeptide on the yield of bacillomycin D,rapCgene in fmbJ was successfully deleted by the marker-free method. Finally, it was found that the deletion ofrapCgene in fmbJ significantly improved bacillomycin D production from 240.7 ± 18.9 to 360.8 ± 30.7 mg/L, attributed to the increased the expression of bacillomycin D synthesis-related genes through enhancing the transcriptional level ofcomA,comP, andphrC. These results showed that the production of bacillomycin D inB. amyloliquefaciensfmbJ might be regulated by the RapC–PhrC system. The findings are expected to advance further agricultural application ofBacillusspp. as a promising source of natural bioactive compounds.
