Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.
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酿酒酵母 6 个最小染色体的物理图谱，分辨率为 2.6 千碱基对。
DOI：
10.1093/genetics/134.1.81
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Olson,MV
中科院分区：
文献类型：
--
作者：
Riles,L;Dutchik,JE;Baktha,A;McCauley,BK;Thayer,EC;Leckie,MP;Braden,VV;Depke,JE;Olson,MV
给出了酿酒酵母6条最小染色体的物理图谱。按大小排列，它们是染色体I、VI、III、IX、V和VIII，包括2.49兆碱基的DNA对。这些图谱是基于对一组重叠的lambda和Cosmid克隆的分析得出的。相邻克隆之间的重叠由EcoRI和HindIII共同作用产生的共享限制性片段识别。定位的裂解位点之间的平均间距为2.6kb。六条染色体中有五条是从一端到另一端的，没有间断；在第九染色体的图谱中只有一个内部缺口。报道的图谱覆盖了六条染色体上约97%的DNA；几乎所有缺失的片段都是端粒。这些图谱与A.J.林克和M.V.奥尔森之前发表的酵母基因组SfiI/Noti图谱完全交叉相关。它们还与酵母遗传图谱在51个座位上进行了交叉关联。
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae are presented. In order of increasing size, they are chromosomes I, VI, III, IX, V and VIII, comprising 2.49 megabase pairs of DNA. The maps are based on the analysis of an overlapping set of lambda and cosmid clones. Overlaps between adjacent clones were recognized by shared restriction fragments produced by the combined action of EcoRI and HindIII. The average spacing between mapped cleavage sites is 2.6 kb. Five of the six chromosomes were mapped from end to end without discontinuities; a single internal gap remains in the map of chromosome IX. The reported maps span an estimated 97% of the DNA on the six chromosomes; nearly all the missing segments are telomeric. The maps are fully cross-correlated with the previously published SfiI/NotI map of the yeast genome by A. J. Link and M. V. Olson. They have also been cross-correlated with the yeast genetic map at 51 loci.