Patterns of spon1b:GFP expression during early zebrafish brain development
Patterns of spon1b:GFP expression during early zebrafish brain development
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DOI：
10.1186/s13104-019-4876-x
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发表时间：
2020-01-07
影响因子：
1.8
通讯作者：
Akle, Veronica
中科院分区：
文献类型：
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作者：
Agudelo-Duenas, Nathalie;Forero-Shelton, Manu;Akle, Veronica
目的：f -spondin是一组进化保守的脊椎动物细胞外基质蛋白。它在胚胎底板中高度表达，能与外基质结合，促进神经元的生长。我们小组先前报道了成年斑马鱼大脑中F-spondin表达模式的表征。然而，鉴于其在发育过程中的重要性，我们的目标是利用光片荧光显微镜（LSFM）获得由spon1b启动子驱动的绿色荧光蛋白（GFP）在转基因Tg（spon1b:GFP）系发育中的斑马鱼大脑中表达的详细描述。结果在受精后22 ~ 96 h的活胚胎中获得的图像证实了我们之前关于在端脑和间脑（嗅球、束带、视顶盖、内侧纵束核）中存在海绵1b:GFP表达细胞的报道，并在垂体、背吻侧簇和腹吻侧簇中发现了新的海绵1b:GFP群体。LSFM使得在发育过程中跟踪细胞迁移模式的动态成为可能。结论：GFP幼虫的表达模式始于发育早期，在发育中的大脑特定神经元结构中与感觉-运动调节有关。转基因Tg（spon1b:GFP）系的LSFM评价为表征GFP在体内的表达模式提供了一种有效的方法。
ObjectiveF-spondin is part of a group of evolutionarily conserved extracellular matrix proteins in vertebrates. It is highly expressed in the embryonic floor plate, and it can bind to the ECM and promote neuronal outgrowth. A characterization of F-spondin expression patterns in the adult zebrafish brain was previously reported by our group. However, given its importance during development, we aimed to obtain a detailed description of green fluorescent protein (GFP) expression driven by the spon1b promotor, in the developing zebrafish brain of the transgenic Tg(spon1b:GFP) line, using light sheet fluorescence microscopy (LSFM).ResultsImages obtained in live embryos from 22 to 96 h post fertilization confirmed our earlier reports on the presence of spon1b:GFP expressing cells in the telencephalon and diencephalon (olfactory bulbs, habenula, optic tectum, nuclei of the medial longitudinal fasciculus), and revealed new spon1b:GFP populations in the pituitary anlage, dorso-rostral cluster, and ventro-rostral cluster. LSFM made it possible to follow the dynamics of cellular migration patterns during development.Conclusionsspon1b:GFP larval expression patterns starts in early development in specific neuronal structures of the developing brain associated with sensory-motor modulation. LSFM evaluation of the transgenic Tg(spon1b:GFP) line provides an effective approach to characterize GFP expression patterns in vivo.