A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity
A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity
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DOI：
10.1007/s10858-007-9149-7
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发表时间：
2007-05-01
影响因子：
2.7
通讯作者：
Kay, Lewis E.
中科院分区：
文献类型：
--
作者：
Lundstrom, Patrik;Vallurupalli, Pramodh;Kay, Lewis E.
描述了用于记录蛋白质中C-13选择性标记甲基的单量子C-13-甲基弛豫色散谱的脉冲序列，相对于现有的初始磁化来自C-13极化的方法，该脉冲序列在灵敏度上提供了显着改进。利用H-1自旋的极化和H-1 -> - C-13 ->的H-1型磁化转移，获得了新实验的灵敏度增益。它的实用性已被应用于涉及三种不同的蛋白质系统，其分子量范围从8到28 kDa，使用许多不同的选择性标记方法生产。在所有情况下，来自C-13 -> H-1和H-1 -> C-13 -> H-1类实验的交换参数都很一致，使用新方案实现的灵敏度增益在1.7到4倍之间。
A pulse sequence is described for recording single-quantum C-13-methyl relaxation dispersion profiles of C-13-selectively labeled methyl groups in proteins that offers significant improvements in sensitivity relative to existing approaches where initial magnetization derives from C-13 polarization. Sensitivity gains in the new experiment are achieved by making use of polarization from H-1 spins and H-1 -> C-13 -> H-1 type magnetization transfers. Its utility has been established by applications involving three different protein systems ranging in molecular weight from 8 to 28 kDa, produced using a number of different selective labeling approaches. In all cases exchange parameters from both C-13 -> H-1 and H-1 -> C-13 -> H-1 classes of experiment are in good agreement, with gains in sensitivity of between 1.7 and 4-fold realized using the new scheme.