Recent worldwide expansion of Nosema ceranae (Microsporidia) in Apis mellifera populations inferred from multilocus patterns of genetic variation
Recent worldwide expansion of Nosema ceranae (Microsporidia) in Apis mellifera populations inferred from multilocus patterns of genetic variation
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DOI：
10.1016/j.meegid.2015.01.002
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发表时间：
2015-04-01
影响因子：
3.2
通讯作者：
Maside, X.
中科院分区：
文献类型：
--
作者：
Gomez-Moracho, T.;Bartolome, C.;Maside, X.
人们发现，蜜蜂微孢子虫感染蜜蜂群体的频率越来越高，现在它对全世界蜜蜂的健康和长期生存构成了重大威胁。然而，到目前为止，人们对这种寄生虫的群体遗传学知之甚少。在这里，我们描述了来自世界各地的分离株集合中三个基因组位点的遗传变异模式。我们的主要发现是：(i) 遗传多态性水平（pi(s) 接近 1%）在其分布范围内没有显着变化，(ii) 有大量证据表明单倍型之间存在重组，(iii) 观察到的遗传方差的最佳部分对应于蜂群内的差异（高达总方差的 88%），(iv) 从亚洲蜜蜂（中华蜜蜂和小蜜蜂）收集的寄生虫与获得的寄生虫表现出显着差异来自意大利蜜蜂的样本（总方差的 8-16%，p < 0.01）和 (v) 在从意大利蜜蜂获得的样本中，低频变异显着超过中性预期，但不是从亚洲蜜蜂获得的样本。总体而言，这些结果与近期蜜蜂花在整个蜜蜂菌落中的定植和快速扩张是一致的。 (C) 2015 Elsevier B.V. 保留所有权利。
Nosema ceranae has been found infecting Apis mellifera colonies with increasing frequency and it now represents a major threat to the health and long-term survival of these honeybees worldwide. However, so far little is known about the population genetics of this parasite. Here, we describe the patterns of genetic variation at three genomic loci in a collection of isolates from all over the world. Our main findings are: (i) the levels of genetic polymorphism (pi(s) approximate to 1%) do not vary significantly across its distribution range, (ii) there is substantial evidence for recombination among haplotypes, (iii) the best part of the observed genetic variance corresponds to differences within bee colonies (up to 88% of the total variance), (iv) parasites collected from Asian honeybees (Apis cerana and Apis florea) display significant differentiation from those obtained from Apis mellifera (8-16% of the total variance, p < 0.01) and (v) there is a significant excess of low frequency variants over neutral expectations among samples obtained from A. mellifera, but not from Asian honeybees. Overall these results are consistent with a recent colonization and rapid expansion of N. ceranae throughout A. mellifera colonies. (C) 2015 Elsevier B.V. All rights reserved.