Protein synthesis by isolated pachytene spermatocytes in the absence of Sertoli cells.
Protein synthesis by isolated pachytene spermatocytes in the absence of Sertoli cells.
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在缺乏支持细胞的情况下，分离的粗线期精母细胞的蛋白质合成。
DOI：
10.1002/jez.1402330217
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. J. Molen
中科院分区：
文献类型：
--
作者：
Nicolet H. P. M. Jutte;R. Jansen;J. Grootegoed;F. Rommerts;H. J. Molen
将分离的大鼠粗线期精母细胞在补充有丙酮酸盐和乳酸盐的化学成分确定的培养基中孵育，已知丙酮酸盐和乳酸盐是这些生殖细胞的必需能量底物。蛋白质合成的分离细胞进行了研究，通过二维聚丙烯酰胺凝胶电泳。粗线期精母细胞在（35 S）蛋氨酸存在下孵育0-2 h或分离后24-26 h合成的（35 S）标记蛋白的电泳图谱几乎完全相同。新合成的蛋白质的新鲜分离的精母细胞和精子细胞的模式，但是，除了许多共同的生精细胞类型的蛋白质，显示了几个阶段特异性蛋白质。因此，可以得出结论，在没有支持细胞的情况下，但在存在适当的能量来源的情况下，粗线期精母细胞在孵育24小时期间可以维持蛋白质合成的阶段特异性模式。
Isolated rat pachytene spermatocytes were incubated in chemically defined medium supplemented with pyruvate and lactate, which are known to be essential energy substrates for these germ cells. Protein synthesis by the isolated cells was investigated by means of two-dimensional polyacrylamide gel electrophoresis. The electrophoretic patterns of (35S)-labeled proteins, synthesized by the pachytene spermatocytes during incubation in the presence of (35S)methionine either from 0-2 h or from 24-26 h after isolation, were almost completely identical. The patterns of newly synthesized proteins of freshly isolated spermatocytes and spermatids, however, showed several stage-specific proteins in addition to many proteins common to both spermatogenic cell types. Hence, it was concluded that a stage-specific pattern of protein synthesis can be maintained by pachytene spermatocytes during incubation for a period of 24 h in the absence of Sertoli cells but in the presence of a proper energy source.
哺乳动物精子发生过程中的基因表达。
影响因子：
3.6
作者：
Stern,L;Gold,B;Hecht,NB
通讯作者：
Hecht,NB
影响因子：
3.6
作者：
MEISTRICH, ML;LONGTIN, J;MACE, ML
通讯作者：
MACE, ML