Novel in situ multi-level analysis of structural-mechanical relations in a bioinspired polyurethane-based tissue model
Novel in situ multi-level analysis of structural-mechanical relations in a bioinspired polyurethane-based tissue model
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DOI：
10.1016/j.mtadv.2021.100184
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发表时间：
2021-12
影响因子：
10
通讯作者：
J. Mo;N. Leung;Priyank Gupta;B. Zhu;E. Velliou;T. Sui
中科院分区：
文献类型：
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作者：
J. Mo;N. Leung;Priyank Gupta;B. Zhu;E. Velliou;T. Sui
在这篇手稿中，我们首次阐明了我们最近开发的基于生物启发聚氨酯的胰腺组织模型中存在的亚结构机制。模型的不同蛋白质涂层，即，检测胶原和纤连蛋白。更具体地说，通过结合实时同步加速器X射线散射技术和共聚焦激光扫描显微镜进行分析，以量化未涂覆的聚氨酯（PU）和蛋白质涂覆的PU的结构变化，以及在原位微机械测试期间在微米，纳米和晶格长度尺度上发生的时间分辨结构重组。我们证明了纤连蛋白-PU改性后层状水平的刚度明显增加，这与层状和层间内聚力的力学变化有关。在这种基于多聚酶的胰腺癌组织模型中的结构-机械关系的这种多层次分析为设计机械上稳健的具有成本效益的组织模型提供了机会，不仅用于基础研究，而且用于治疗筛选。
In this manuscript, we elucidated, for the first time, the substructural mechanisms present in our recently developed bioinspired polyurethane-based pancreatic tissue models. Different protein coatings of the model, i.e., collagen and fibronectin were examined. More specifically, analysis took place by combined real-time synchrotron X-ray scattering techniques and confocal laser scanning microscopy, to quantify the structural alteration of uncoated-polyurethane (PU) and protein-coated PU as well as the time-resolved structural reorganisation occurring at the micro-, nano- and lattice length scales during in situ micromechanical testing. We demonstrate that a clear increase of stiffness at the lamellar level following the fibronectin-PU modification, which is linked to the changes in the mechanics of the lamellae and interlamellar cohesion. This multi-level analysis of structural-mechanical relations in this polyurethane-based pancreatic cancer tissue model opens an opportunity in designing mechanically robust cost-effective tissue models not only for fundamental research but also for treatment screening.