Transcriptomics-based validation of the relatedness of heterogeneous nuclear ribonucleoproteins to chronic lymphocytic leukemia as potential biomarkers of the disease aggressiveness
Transcriptomics-based validation of the relatedness of heterogeneous nuclear ribonucleoproteins to chronic lymphocytic leukemia as potential biomarkers of the disease aggressiveness
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DOI：
10.15537/smj.2019.4.23380
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发表时间：
2019-04-01
影响因子：
1.6
通讯作者：
Alsagaby, Suliman A.
中科院分区：
文献类型：
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作者：
Alsagaby, Suliman A.
目的：使用独立的转录组学数据集的癌症患者的预后信息从公共存储库验证我们以前描述的慢性淋巴细胞白血病（CLL）相关蛋白的相关性在转录水平（mRNA）的预后CLL.Methods：这是一个验证研究，在Majmaah大学，沙特阿拉伯王国之间进行的一月-2017和七月-2018。使用来自Gene Expression Omnibus（GEO）的CLL转录组学的两个独立数据集，以及首次治疗时间（TTFT）数据（GSE 39671; 130例患者）和总生存期（OS）信息（GSE 22762; 107例患者）进行验证分析。为了进一步研究感兴趣的转录本与其他肿瘤的相关性，使用了来自癌症基因组学图谱（TCGA）的具有预后信息的癌症转录组学的6个独立数据集（1865名患者）。结果：9个CLL相关蛋白的转录表达预测TTFT，7个CLL相关蛋白的mRNA表达预测CLL患者的OS（P
Objectives: To use independent transcriptomics data sets of cancer patients with prognostic information from public repositories to validate the relevance of our previously described chronic lymphocytic leukemia (CLL)-related proteins at the level of transcription (mRNA) to the prognosis of CLL.Methods: This is a validation study that was conducted at Majmaah University, Kingdom of Saudi Arabia between January-2017 and July-2018. Two independent data sets of CLL transcriptomics from Gene Expression Omnibus (GEO) with time-to-first treatment (TTFT) data (GSE39671; 130 patients) and information about overall survival (OS) (GSE22762; 107 patients) were used for the validation analyses. To further investigate the relatedness of a transcript of interest to other neoplasms, 6 independent data sets of cancer transcriptomics with prognostic information (1865 patients) from the cancer genomics atlas (TCGA) were used. Pathway-enrichment analyses were conducted using Reactome; and correlation analyses of gene expression were performed using Pearson score.Results: Nine of the CLL-related proteins exhibited transcript expression that predicted TTFT and 7 of the CLL-related proteins showed mRNA levels that predicted OS in CLL patients (p