Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
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DOI：
10.1093/nar/gkl542
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发表时间：
2006-09-01
影响因子：
14.9
通讯作者：
Busby, Stephen J. W.
中科院分区：
文献类型：
--
作者：
Grainger, David C.;Hurd, Douglas;Busby, Stephen J. W.
大肠杆菌染色体被浓缩成一种不明确的结构，称为类核。类核相关的 DNA 结合蛋白参与维持这种结构并介导染色体压缩。我们利用染色质免疫沉淀和高密度微阵列来研究 FIS、H-NS 和 IHF 三种蛋白质在体内大肠杆菌基因组中的结合。我们的结果表明，这些蛋白质的分布偏向于基因组的基因间部分，并且结合谱重叠。因此，一些目标与结合的 FIS、H-NS 和 IHF 的组合相关。此外，许多与FIS和H-NS相关的区域也与RNA聚合酶相关。
The Escherichia coli chromosome is condensed into an ill-defined structure known as the nucleoid. Nucleoid-associated DNA-binding proteins are involved in maintaining this structure and in mediating chromosome compaction. We have exploited chromatin immunoprecipitation and high-density microarrays to study the binding of three such proteins, FIS, H-NS and IHF, across the E.coli genome in vivo. Our results show that the distribution of these proteins is biased to intergenic parts of the genome, and that the binding profiles overlap. Hence some targets are associated with combinations of bound FIS, H-NS and IHF. In addition, many regions associated with FIS and H-NS are also associated with RNA polymerase.