MTHFR C677T, A1298C and MS A2756G Gene Polymorphisms and Male Infertility Risk in a Chinese Population: A Meta-Analysis.
MTHFR C677T, A1298C and MS A2756G Gene Polymorphisms and Male Infertility Risk in a Chinese Population: A Meta-Analysis.
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DOI：
10.1371/journal.pone.0169789
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Dong Q
中科院分区：
文献类型：
--
作者：
Ren Z;Ren P;Yang B;Fang K;Ren S;Liao J;Liu S;Liu L;Peng Z;Dong Q
亚甲基四氢叶酸还原酶基因（MTHFR C677 T和A1298 C）和甲硫氨酸合成酶基因（MS A2756 G）多态性与几个种族人群的男性不育风险相关。尽管有几项研究在中国人群中评估了这些关联，但其样本量小和结果不一致，无法得出强有力的结论。因此，本荟萃分析与已发表的研究，以评估的三个单核苷酸多态性（SNPs）与中国人群中的男性不育症的关联。我们对PubMed、Embase、Web of Science、中国知网（CNKI）、中国生物医学文献（CBM）、VIP和中文文献（万方）数据库进行了检索，截止日期为2016年5月31日。根据异质性分析结果，采用随机效应模型或固定效应模型，使用比值比（OR）和95%置信区间（95%CI）评估关联强度。采用敏感性分析验证Meta分析的可靠性和稳定性。共有9项研究，包括1，713例病例和1，104例对照，被纳入荟萃分析。汇总结果表明，在等位基因模型中，MTHFR C667 T多态性与中国人群男性不育风险增加显着相关（T vs. C：OR = 1.47，95%CI = 1.32-1.63），优势模型（TT + CT vs. CC：OR = 1.51，95%CI = 1.30-1.77），相加模型（TT vs. CC：OR = 2.08，95%CI = 1.68-2.58）和隐性模型（TT vs. CT+CC：OR = 1.58，95%CI = 1.31-1.90），而MTHFR A1298 C和MS A2756 G多态性不是危险因素。研究中的任何基因型对比均无显著异质性。敏感性分析表明，本次荟萃分析的结果相对稳定。MTHFR C667 T多态性可能与男性不育的遗传易感性有关，而MTHFR A1298 C和MS A2756 G多态性可能与男性不育无关。需要更大样本量的研究和代表性的基于人群的病例和匹配良好的对照来验证我们的结果。
Methylenetetrahydrofolate reductase gene (MTHFR C677T and A1298C) and methionine synthase gene (MS A2756G) polymorphisms have shown an association with male infertility risk in several ethnic populations. Although several studies have evaluated these associations in Chinese populations, their small sample sizes and inconsistent outcomes have prevented strong conclusions. Therefore, the present meta-analysis was performed with published studies to evaluate the associations of the three single nucleotide polymorphisms (SNPs) and male infertility in a Chinese population. We conducted a search of PubMed, Embase, Web of Science, Chinese National Knowledge Infrastructure (CNKI), China biology medical literature (CBM), VIP, and Chinese literature (Wan Fang) databases up to May 31, 2016. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were used to assess the strength of associations with a random-effect model or a fixed-effect model based on the heterogeneity analysis results. Sensitivity analysis was used to confirm the reliability and stability of the meta-analysis. A total of nine studies, including 1,713 cases and 1,104 controls, were included in the meta-analysis. The pooled results indicated that the MTHFR C667T polymorphism was significantly associated with increased risk of male infertility in the Chinese population in the allele model (T vs. C: OR = 1.47, 95%CI = 1.32–1.63), the dominant model (TT + CT vs. CC: OR = 1.51, 95%CI = 1.30–1.77), the additive model (TT vs. CC: OR = 2.08, 95%CI = 1.68–2.58) and the recessive model (TT vs. CT+CC: OR = 1.58, 95%CI = 1.31–1.90), whereas the MTHFR A1298C and MS A2756G polymorphisms were not risk factors. There was no significant heterogeneity in any genotype contrasts among the studies. The sensitivity analysis indicated that the results of this meta-analysis were relatively stable. This study suggests that the MTHFR C667T polymorphism may contribute to the genetic susceptibility to male infertility in the Chinese population, whereas MTHFR A1298C and MS A2756G polymorphisms may be unrelated to male infertility. Studies with larger sample sizes and representative population-based cases and well-matched controls are needed to validate our results.