Development of a Vivid FRET System Based on a Highly Emissive dG-dC Analogue Pair
Development of a Vivid FRET System Based on a Highly Emissive dG-dC Analogue Pair
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DOI：
10.1002/chem.201701118
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发表时间：
2017-06-01
影响因子：
4.3
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Han, Ji Hoon;Yamamoto, Seigi;Sugiyama, Hiroshi
报道了一种新型的高发射同晶碱基类似物的Forster共振能量转移（FRET）体系。FRET对由作为能量供体的2-氨基硫代-无[3，4-d]嘧啶G-模拟脱氧核糖核苷（thdG）和作为能量受体的1，3-二氮杂-2-氧代吩噻嗪（tC）组成。通过将（th）dG和tC系统地掺入DNA序列中来控制供体和受体之间的距离和取向，以研究FRET效率。这是第一个沃森-克里克碱基配对FRET对产生生动的颜色。此外，这种基于核酸的FRET对用于监测DNA构象，并实现了B-Z转换的可视化。
A new type of Forster Resonance Energy Transfer (FRET) system using highly emissive isomorphic nucleobase analogues is reported. The FRET pair consists of 2-aminothie-no[ 3,4-d] pyrimidine G-mimic deoxyribonucleoside (thdG) as an energy donor and 1,3-diaza-2-oxophenothiazine (tC) as an energy acceptor. The distance and orientation between donor and acceptor was controlled by systematic incorporation of (th)dG and tC into DNA sequences to investigate the FRET efficiencies. This is the first Watson-Crick base-pairable FRET pair to produce vivid colors. In addition, this nucleic acid-based FRET pair was used to monitor DNA conformation and achieved visualization of the B-Z transition.