Analysis of commercial kidney stone probiotic supplements.
Analysis of commercial kidney stone probiotic supplements.
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DOI：
10.1016/j.urology.2014.11.013
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发表时间：
2015-03
期刊：
影响因子：
2.1
通讯作者：
Knight J
中科院分区：
文献类型：
--
作者：
Ellis ML;Shaw KJ;Jackson SB;Daniel SL;Knight J
检查由™PRO Lab，Ltd，多伦多，加拿大销售的益生菌补充剂和购自Sanzyme Ltd，Hyderabad，印度的Oxalo™胶囊中的草酸根形成菌的水平，并测量这些制剂体外降解草酸根的能力。益生菌补充剂和O.在含有草酸盐的多种培养基中培养产甲酸菌。OD 595用于测量细菌生长，离子色谱法用于测量培养基中草酸盐的损失。O.在PCR中使用草酸脱羧酶基因（oxc）的产甲酸杆菌特异性和简并乳酸杆菌引物。在不同培养基中孵育益生菌补充剂不会导致降解乳酸盐的生物体生长。PCR表明没有携带oxc基因的微生物。培养物和16S rRNA基因测序表明™PRO实验室补充剂含有活的乳酸乳球菌乳酸亚种。1），而Oxalo™含有几种芽孢杆菌属物种和植物乳杆菌。由™PRO Lab，Ltd和Sanzyme Ltd在互联网上销售的益生菌补充剂不含可识别的O。草酸钙肾结石患者可能不需要使用产甲酸菌或可降解草酸盐的活菌，因此不太可能受益。
To examine the levels of Oxalobacter formigenes in probiotic supplements marketed by ™PRO Lab, Ltd, Toronto, Canada, and capsules of Oxalo™ purchased from Sanzyme Ltd, Hyderabad, India, and to measure the ability of these preparations to degrade oxalate in vitro. Probiotic supplements and pure cultures of O. formigenes were cultured in a number of media containing oxalate. OD595 was used to measure bacterial growth and ion chromatography was used to measure loss of oxalate in culture media. O. formigenes specific and degenerate Lactobacillus primers to the oxalate decarboxylase gene (oxc) were used in PCR. Incubating probiotic supplements in different media did not result in growth of oxalate-degrading organisms. PCR indicated the absence of organisms harboring the oxc gene. Culture and 16S rRNA gene sequencing indicated the ™PRO Lab supplement contained viable Lactococcus lactis subsp. lactis (GenBank accession no. KJ095656.1), while Oxalo™ contained several Bacillus species and Lactobacillus plantarum. The probiotic supplement sold over the internet by ™PRO Lab, Ltd and Sanzyme Ltd did not contain identifiable O. formigenes or viable oxalate-degrading organisms, and they are unlikely to be of benefit to calcium oxalate kidney stone patients.
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影响因子：
3.2
作者：
Costa, T;Steil, L;Henriques, AO
通讯作者：
Henriques, AO
影响因子：
13.6
作者：
Kaufman, David W.;Kelly, Judith P.;Cave, David R.
通讯作者：
Cave, David R.
影响因子：
168.9
作者：
Sidhu, H;Hoppe, B;Peck, AB
通讯作者：
Peck, AB
影响因子：
2.8
作者：
ALLISON, MJ;DAWSON, KA;FOSS, JG
通讯作者：
FOSS, JG
影响因子：
6.1
作者：
Hoppe, Bernd;Groothoff, Jaap W.;Milliner, Dawn
通讯作者：
Milliner, Dawn