Oncogenic role of Epstein-Barr virus-encoded RNAs in Burkitt's lymphoma cell line Akata
Oncogenic role of Epstein-Barr virus-encoded RNAs in Burkitt's lymphoma cell line Akata
复制标题
DOI：
10.1128/jvi.73.12.9827-9831.1999
复制
发表时间：
1999-12-01
影响因子：
5.4
通讯作者：
Takada, K
中科院分区：
文献类型：
--
作者：
Komano, J;Maruo, S;Takada, K
我们以前的报道表明，EB病毒(Epstein-Barr Virus，EBV)与Burkitt淋巴瘤细胞株Akata(N，Shimizu，A.Tanabe-Tochikura，Y.Kuroiwa和K，Takada，J，Virol，68：6069-6073,1994；我们将EBV编码的小RNA(EBERs)导入EBV阴性的AKATA克隆，使其在软琼脂中的生长能力、在SCID小鼠中的致瘤性、对凋亡诱导剂的抗性以及在EBV阴性亚克隆中缺失的bcl2癌蛋白的表达上调)，这是首次报道EBV编码的小RNA(EBERs)在EBV阴性的Akata细胞中具有癌基因功能的证据。
Our previous reports indicated that Epstein-Barr virus (EBV) contributes to the malignant phenotype and resistance to apoptosis in Burkitt's lymphoma (BL) cell line Akata (N, Shimizu, A. Tanabe-Tochikura, Y. Kuroiwa, and K, Takada, J, Virol, 68:6069-6073, 1994; J, Komano, M, Sugiura, and K, Takada, J, Virol, 72:9150-9156, 1998), Here we report that the EBV-encoded small RNAs (EBERs) are responsible for these phenotypes, Transfection of the EBER genes into EBV-negative Akata clones restored the capacity for growth in soft agar, tumorigenicity in SCID mice, resistance to apoptotic inducers, and upregulated expression of bcl-2 oncoprotein that were originally retained in parental EBV-positive Akata cells and lost in EBV-negative subclones, This is the first report which provides evidence that virus-encoded RNAs (EBERs) have oncogenic functions in BL cells.