Three cytochrome P450 CYP4 family genes regulated by the CncC signaling pathway mediate phytochemical susceptibility in the red flour beetle, Tribolium castaneum
Three cytochrome P450 CYP4 family genes regulated by the CncC signaling pathway mediate phytochemical susceptibility in the red flour beetle, Tribolium castaneum
复制标题
受 CncC 信号通路调节的三个细胞色素 P450 CYP4 家族基因介导赤拟谷盗的植物化学敏感性
DOI：
10.1002/ps.6991
复制
发表时间：
2022
影响因子：
4.1
通讯作者：
Bin Li
中科院分区：
文献类型：
--
作者：
Yonglei Zhang;Runnan Yu;Jing Tang;Liheng Du;Yihan Wang;Jiatao Wang;Linsu Liu;Shanshan Gao;Bin Li
昆虫细胞色素P450单加氧酶（P450）在植物化学物质代谢和耐受性中起关键作用。赤拟谷盗中有3个P450基因（TcCYP 4 Q3、TcCYP 4 Q5、TcCYP 4 Q7）与丁香酚的反应有关。然而，这些P450基因对丁香酚的反应机制尚不清楚。结果：时空表达谱显示，TcCYP 4 Q3和TcCYP 4 Q5在晚期成体中表达最高，而TcCYP 4 Q7主要在晚期幼虫中表达;这3个P450基因主要在幼虫的脂肪体中表达。此外，这三个P450基因的表达显着上调后，暴露于丁香酚，和消耗增强了甲虫对丁香酚的敏感性。有趣的是，针对CncC基因的RNA干扰（RNAi），CncC信号通路的转录因子与响应植物化学物质的昆虫P450的调节，减少这三个P450基因的转录暴露于丁香酚。对CncC信号通路的研究表明，丁香酚可激活CncC信号通路。结论：总之，结果表明这三个P450基因受CncC信号通路的调控，参与了T.植物化学物质。这些发现将有助于开发新的治疗方法来控制害虫。
BACKGROUND.Insect cytochrome P450 monooxygenases (P450s) play a crucial role in phytochemical metabolism and tolerance. Three P450 genes (TcCYP4Q3, TcCYP4Q5, TcCYP4Q7) are associated with the response of eugenol in Tribolium castaneum. However, the responding mechanisms of these P450 genes to eugenol remain unknown..RESULTS.Here, spatiotemporal expression profiling revealed that TcCYP4Q3 and TcCYP4Q5 were most highly expressed in late adult, while TcCYP4Q7 was predominantly expressed in late larva; and all of these three P450 genes were mainly expressed in the fat body of larvae. Furthermore, the expressions of these three P450 genes were significantly up-regulated after exposure to eugenol, and depletion of them enhanced the susceptibility of beetles to eugenol. Interestingly, RNA interference (RNAi) against the CncC gene, a transcription factor of CncC signaling pathway associated with regulation of insect P450s in response to phytochemicals, reduced the transcripts of these three P450 genes following exposure to eugenol. Investigation of CncC signaling pathway showed that this pathway could be activated by eugenol..CONCLUSION.Altogether, the results indicate that these three P450 genes are regulated by CncC signaling pathway to participate in the susceptibility of T. castaneum to phytochemicals. These findings will aid implications for the development of novel therapeutics to control pest.