Expression of 53BP2 and ASPP2 proteins from TP53BP2 gene by alternative splicing.
Expression of 53BP2 and ASPP2 proteins from TP53BP2 gene by alternative splicing.
复制标题
DOI：
10.1016/j.bbrc.2004.01.079
复制
发表时间：
2004-03
影响因子：
3.1
通讯作者：
N. Takahashi;Shinya Kobayashi;Xu Jiang;Koji Kitagori;K. Imai;Y. Hibi;T. Okamoto
中科院分区：
文献类型：
--
作者：
N. Takahashi;Shinya Kobayashi;Xu Jiang;Koji Kitagori;K. Imai;Y. Hibi;T. Okamoto
p53 结合蛋白 2 (53BP2) 最初被鉴定为 p53 的相互作用蛋白，随后的研究表明它还与 Bcl-2 和 NF-κB p65 亚基相互作用。我们之前发现编码53BP2蛋白的TP53BP2基因是单拷贝基因，并且已被定位到1号染色体长臂q42.1处。随后的研究表明，TP53BP2编码两种蛋白质，53BP2和ASPP2，分别含有1005个和1128个氨基酸。 ASPP2 在 53BP2 的 N 末端含有额外的 123 个氨基酸。在这项研究中，我们检查了 TP53BP2 转录本的基因组组织，发现它通过在各种细胞系和组织中的选择性剪接编码两种 mRNA 种类，带有 (53BP2) 或不带有外显子 3 (ASPP2)。因此，我们建议将这些蛋白质分别称为 53BP2S（短）和 53BP2L（长）。
The p53 binding protein 2 (53BP2) has been initially identified as an interacting protein to p53 and subsequent studies have shown that it also interacts with Bcl-2 and NF-κB p65 subunit. We have previously found that the TP53BP2 gene encoding 53BP2 protein is a single copy gene and has been mapped to the long arm of chromosome 1 at q42.1. The subsequent studies revealed that TP53BP2 encodes two proteins, 53BP2 and ASPP2, of 1005 and 1128 amino acids, respectively. ASPP2 contains additional 123 amino acids to the N-terminus of 53BP2. In this study, we have examined the genomic organization of TP53BP2 transcripts and found that it encodes two mRNA species, either with (53BP2) or without exon 3 (ASPP2), by alternative splicing in various cell lines and tissues. Thus, we propose to call these proteins as 53BP2S (short) and 53BP2L (long), respectively.