The effect of interferon treatment on 14 human colorectal cancer cell lines: growth and carcinoembryonic antigen secretion in vitro.
The effect of interferon treatment on 14 human colorectal cancer cell lines: growth and carcinoembryonic antigen secretion in vitro.
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干扰素治疗对 14 种人结直肠癌细胞系的影响：体外生长和癌胚抗原分泌。
DOI：
10.1089/jir.1990.10.579
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Thomas,P
中科院分区：
文献类型：
--
作者：
Toth,CA;Thomas,P
体外研究了干扰素 (IFN) 对 14 种已建立的人结直肠癌细胞系的生长抑制和癌胚抗原 (CEA) 分泌的影响。细胞系的特征在于形态分化、CEA 产生水平和生长速率。所有细胞系均用重组人 IFN（α、β 和 γ）进行体外处理，并测定处理对生长速率和 CEA 分泌的影响。每个细胞系都表现出独特的生长抑制模式，与分化程度、CEA 产生水平和生长速率无关。 IFN-β 治疗并未增加所研究的任何细胞系中 CEA 的分泌。 IFN-α 和 IFN-γ 导致 CEA 产量增加（增加 2 至 81 倍），主要是在中度至良好分化的细胞系中。 IFN-γ 是比 IFN-α 更有效的 CEA 分泌增强诱导剂。分化程度较低的细胞系不产生 CEA，并且不能被任何 IFN 诱导产生 CEA。
The effect of interferons (IFNs) on growth inhibition and carcinoembryonic antigen (CEA) secretion by 14 established human colorectal carcinoma cell lines was studiedin vitro. The cell lines were characterized by morphologic differentiation, level of CEA production, and rate of growth. All cell lines were treatedin vitrowith recombinant human IFN (α, β, and γ) and the effect of treatment on growth rate and CEA secretion determined. Each cell line exhibited an individual pattern of growth inhibition that was independent of degree of differentiation, level of CEA production, and rate of growth. IFN-β treatment did not increase CEA secretion in any of the cell lines studied. IFN-α and IFN-γ resulted in increased CEA production (2- to 81-fold increase) primarily in the moderately to well-differentiated cell lines. IFN-γ was a more potent inducer of enhanced CEA secretion than IFN-α. The more poorly differentiated cell lines did not produce CEA and could not be induced to do so by any of the IFNs.