Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes
Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes
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DOI：
10.1002/cphc.200500202
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发表时间：
2006-01-16
期刊：
影响因子：
2.9
通讯作者：
Rossier, J
中科院分区：
文献类型：
--
作者：
Amatore, C;Arbault, S;Rossier, J
一氧化氮是一种重要的生物信使，它尤其能诱导血管周围平滑肌细胞松弛，从而控制血液流动。这种机制对大脑功能至关重要，其精细调控（称为功能性充血）被认为是由某些可能释放一氧化氮脉冲的神经元来实现的。本研究的目的是检验镀铂碳纤维微电极（尖端直径5 - 7μm）在直接原位电化学检测神经元释放到离体小脑切片中的一氧化氮方面的优势。在体外对浓度为50nm至1mm的一氧化氮溶液确定了镀铂碳纤维微电极的不同分析特性之后，使用DEA - NONOate溶液对其进行了表征，该溶液会化学分解为一氧化氮，因此可模拟生物样本中一氧化氮浓度瞬时变化的测量。这验证了本方法的有效性，从而展示并讨论了对大鼠小脑切片中神经元释放的一氧化氮进行的直接、原位离体测量。
Nitric oxide is on important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO.. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 mu m tip diameter) for the direct and in situ electrochemical detection of NO. released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO. solutions at 50 nm to 1 mm concentration, they were characterized using DEA-NONOote solutions that chemically decompose into No-. and therefore mimic the measurement of transient variations of NO. concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed.