Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea
Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea
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DOI：
10.1007/s00122-009-1157-4
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发表时间：
2009-12-01
影响因子：
5.4
通讯作者：
Osborn, Thomas C.
中科院分区：
文献类型：
--
作者：
Luis Iniguez-Luy, Federico;Lukens, Lewis;Osborn, Thomas C.
公开的基因组工具可帮助研究人员整合信息并做出新发现。在本文中，我们描述了甘蓝和甘蓝的快速循环、自交亲和系、分子标记和连锁图谱的永生作图种群的发展，并将数据和种质提供给甘蓝研究界。白菜种群由 160 个重组自交 (RI) 系组成，这些重组自交系源自快速循环白菜和黄沙松白菜高度自交系的杂交。甘蓝群体由 155 个双单倍体 (DH) 品系组成，这些品系源自两个 DH 品系（快速循环品系和西兰花）之间的 F1 杂交。总共使用了 120 个 RFLP 探针、146 个 SSR 标记和一种表型性状（花色）来构建两个物种的遗传连锁图谱。白菜图谱由224个分子标记组成，分布在10个连锁群（A1-A10）上，总距离为1125.3 cM，标记密度为5.7 cM/标记。甘蓝遗传图谱由279个分子标记和1个表型标记组成，分布在9个连锁群（C1-C9）上，总距离为891.4 cM，标记密度为3.2 cM/标记。对拟南芥的同线性分析确定了与之前的研究一致的共线基因组块，强化了十字花科保守染色体区域的想法。
Publicly available genomic tools help researchers integrate information and make new discoveries. In this paper, we describe the development of immortal mapping populations of rapid cycling, self-compatible lines, molecular markers, and linkage maps for Brassica rapa and B. oleracea and make the data and germplasm available to the Brassica research community. The B. rapa population consists of 160 recombinant inbred (RI) lines derived from the cross of highly inbred lines of rapid cycling and yellow sarson B. rapa. The B. oleracea population consists of 155 double haploid (DH) lines derived from an F1 cross between two DH lines, rapid cycling and broccoli. A total of 120 RFLP probes, 146 SSR markers, and one phenotypic trait (flower color) were used to construct genetic linkage maps for both species. The B. rapa map consists of 224 molecular markers distributed along 10 linkage groups (A1-A10) with a total distance of 1125.3 cM and a marker density of 5.7 cM/marker. The B. oleracea genetic map consists of 279 molecular markers and one phenotypic marker distributed along nine linkage groups (C1-C9) with a total distance of 891.4 cM and a marker density of 3.2 cM/marker. A syntenic analysis with Arabidopsis thaliana identified collinear genomic blocks that are in agreement with previous studies, reinforcing the idea of conserved chromosomal regions across the Brassicaceae.