Pyrimidine hydrates and dimers in ultraviolet-irradiated tobacco mosaic virus ribonucleic acid.
Pyrimidine hydrates and dimers in ultraviolet-irradiated tobacco mosaic virus ribonucleic acid.
复制标题
紫外线照射的烟草花叶病毒核糖核酸中的嘧啶水合物和二聚体。
DOI：
10.1016/0022-2836(68)90129-0
复制
发表时间：
1968
影响因子：
5.6
通讯作者：
M. Gordon
中科院分区：
文献类型：
--
作者：
G. Small;M. Tao;M. Gordon
建立了一种分离紫外辐射烟草花叶病毒核糖核酸光产物的方法。该方法包括酶水解的紫外线照射的核糖核酸和分离的光产物的纸和离子交换色谱法。五个光产物已被分离：尿苷水合物，环丁烷型二聚体的两种异构形式，和两个未知的光产物。形成的尿苷水合物的量是剂量和溶剂的函数。水合尿苷在水中的生成速率比在氧化氘中的生成速率大。氘同位素效应为2.2。这与病毒核酸生物灭活中观察到的结果一致。嘧啶二聚体和两个未知的光产物的形成速率是相同的，无论是在水中还是在氧化氘中进行照射。结果表明，在低离子强度的溶剂中，紫外光对烟草花叶病毒核糖核酸的灭活主要是由于嘧啶水合物的形成。
A method has been developed to isolate the photoproducts of ultraviolet-irradiated tobacco mosaic virus ribonucleic acid. The method consists of enzymic hydrolysis of the ultraviolet-irradiated ribonucleic acid and isolation of the photoproducts by paper and ion exchange chromatography. Five photoproducts have been isolated: uridine hydrate, two isomeric forms of cyclobutane-type dimers, and two unknown photoproducts. The amount of uridine hydrate formed is a function of dose and the solvent. The rate of formation of uridine hydrate is greater in water than in deuterium oxide. A deuterium isotope effect of 2.2 was observed. This is in agreement with that observed in the biological inactivation of the viral nucleic acid. The rate of formation of pyrimidine dimers and the two unknown photoproducts is the same whether the irradiation is carried out in water or deuterium oxide. The results suggest that the inactivation of tobacco mosaic virus ribonucleic acid by ultraviolet light is mainly due to pyrimidine hydrate formation in solvents of low ionic strength.