Neural interface dynamics following insertion of hydrous iridium oxide microelectrode arrays.
Neural interface dynamics following insertion of hydrous iridium oxide microelectrode arrays.
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插入水合氧化铱微电极阵列后的神经界面动力学。
DOI：
10.1109/iembs.2006.260521
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Kipke,DarylR
中科院分区：
文献类型：
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作者：
Johnson,MatthewD;Langhals,NicholasB;Kipke,DarylR
研究创伤性脑损伤的研究表明，治疗剂存在有效减轻水肿和细胞毒性的“机会之窗”。然而，成功的治疗还依赖于确定血脑屏障破坏的程度，这与过量的细胞外离子，兴奋性氨基酸和血清蛋白浓度有关。以下研究调查了使用pH选择性水合氧化铱微电极来评估插入神经探针后的创伤。铱微电极阵列的电化学活化在酸性（0.5 M H2SO 4）或弱碱性（0.3 M Na 2 HPO 4，pH=8.56）溶液中进行。两种氧化物均表现出超能斯特pH敏感性（分别为-88.5 mV/pH和-77.1 mV/pH），几乎不受其他阳离子的干扰。数据表明，酸生长的氧化物比碱生长的氧化物提供更好的电位稳定性（5小时内σ =2.8对4.9 mV）。将这些电极植入运动皮层和背侧纹状体，在插入过程中和插入后发现了显著的酸中毒。时空pH值分布的变异性包括微尺度的不均匀性沿着探针柄和使用相同的深度和速度连续插入之间的平均pH值响应的显着差异。这一诊断技术对介入治疗具有重要意义，以便更有效地治疗急性外科脑外伤
Studies examining traumatic brain injury have suggested a 'window of opportunity' exists for therapeutic agents to mitigate edema and cellular toxicity effectively. However, successful therapy also relies on identifying the extent of blood-brain barrier disruption, which is associated with excessive extra-cellular concentrations of ions, excitatory amino acids, and serum proteins. The following study investigates the use of pH-selective hydrous iridium oxide microelectrodes to assess trauma following insertion of a neural probe. Electrochemical activation of iridium microelectrode arrays was performed in either acidic (0.5 M H2SO4) or weak basic (0.3 M Na2HPO4, pH=8.56) solutions. Both oxides demonstrated super-Nernstian pH sensitivity (-88.5 mV/pH and -77.1 mV/pH, respectively) with little interference by other cations. Data suggest that acid-grown oxide provides better potential stability than base-grown oxide (sigma=2.8 versus 4.9 mV over 5 hours). Implantation of these electrodes into motor cortex and dorsal striatum revealed significant acidosis during and following insertion. Variability in the spatiotemporal pH profile included micro-scale inhomogeneities along the probe shank and significant differences in the averaged pH response between successive insertions using the same depth and speed. This diagnostic technology has important implications for intervention therapies in order to more effectively treat acute surgical brain trauma