Isolation, mapping, and regulated expression of the gene encoding mouse C-type natriuretic peptide.
Isolation, mapping, and regulated expression of the gene encoding mouse C-type natriuretic peptide.
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小鼠 C 型利尿钠肽编码基因的分离、定位和表达调控。
DOI：
10.1152/ajpheart.1996.271.4.h1565
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Steinhelper
中科院分区：
文献类型：
--
作者：
Huaming Huang;C. G. Acuff;M. Steinhelper
从噬菌体文库中分离编码小鼠C型利钠肽（CNP）的基因组序列，并通过限制性内切酶和序列分析进行表征。小鼠CNP基因（Nppc）包含至少两个外显子和一个内含子，并在5 '侧翼序列中包含几个顺式调控元件。小鼠CNP-22的推导氨基酸序列与其他哺乳动物CNP相同。分析种间回交和重组近交系中的等位基因分布，将Nppc分配到1号染色体。通过核糖核酸酶保护分析在脑、卵巢和子宫中检测到CNP转录物，睾丸和附睾中的水平较低。子宫CNP转录和蛋白质在性未成熟小鼠和发情期成年小鼠中较低，在发情前期增加，但卵巢CNP表达的相似变化无统计学显著性。在小鼠卵巢和子宫中未检测到心房利钠肽和B型利钠肽转录本。因此，CNP基因表达在雌性生殖器官中受组织特异性和诱导机制调节。CNP表达与子宫液含量之间的相关性表明，CNP可以调节小鼠和其他哺乳动物的子宫液平衡。
Genomic sequences encoding mouse C-type natriuretic peptide (CNP) were isolated from bacteriophage libraries and characterized by restriction enzyme and sequence analysis. The mouse CNP gene (Nppc) comprised at least two exons and one intron and included several cis-regulatory elements in the 5'-flanking sequence. The deduced amino acid sequence of mouse CNP-22 was identical to other mammalian CNPs. Analysis of allele distributions in interspecific back-cross and recombinant inbred strains assigned Nppc to chromosome 1. CNP transcripts were detected by ribonuclease protection analysis in brain, ovary, and uterus, with lower levels in testes and epididymus. Uterine CNP transcripts and protein were low in sexually immature mice and adults at estrus and increased at proestrus, but similar variations in ovarian CNP expression were not statistically significant. Atrial natriuretic peptide and B-type natriuretic peptide transcripts were not detected in mouse ovary or uterus. Thus CNP gene expression is regulated by tissue-specific and inducible mechanisms in female reproductive organs. Correlations between CNP expression and uterine fluid content suggest that CNP may regulate uterine fluid balance in mice and other mammals.