Heterofertilization of the opaque-2 endosperm in maize.
Heterofertilization of the opaque-2 endosperm in maize.
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DOI：
10.1111/j.1601-5223.2008.02056.x
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发表时间：
2008-10
期刊：
影响因子：
2.7
通讯作者：
Wenpeng Yang;Yong-lian Zheng;Jing Wu
中科院分区：
文献类型：
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作者：
Wenpeng Yang;Yong-lian Zheng;Jing Wu
玉米籽粒尤其是胚乳性状的优劣直接关系到玉米的品质和产量，因此，籽粒胚乳性状的选择具有重要意义。但异花受精可能影响胚乳表型选择的准确性。本研究的目的是探讨玉米opaque-2（o2）籽粒异受精的频率。以o2为轮回亲本的3个BC（1）F（1）群体和部分BC（1）F（2）后代为材料，研究了胚乳表型和植株基因型。使用简单序列重复（SSR）标记phi 057或phi 112确定植物基因型，所述标记是位于Opaque-2（O2）基因内的序列;目视观察胚乳表型。在BC（1）F（1）群体中，异受精频率为0，约占4.545%。这一结果表明，在回交育种程序中，基于胚乳表型和植物基因型的选择之间可能存在高达5%的差异。分子标记辅助选择是一种更精确的选择方法。此外，我们还讨论了O_2玉米胚乳异受精与O_2位点内变异的关系。
Selection on endosperm traits among individual kernels is very important in maize because the characteristics of the kernel, especially the endosperm, are directly related to quality and yield. However the accuracy of the selection according to endosperm phenotype may be affected by heterofertilization. The objective of this work was to investigate the frequency of heterofertilization of opaque-2 (o2) kernels in maize. Three BC(1)F(1) populations using o2 lines as recurrent parents and some BC(1)F(2) progenies were used to investigate endosperm phenotypes and plant genotypes. The plant genotypes were determined using simple sequence repeat (SSR) markers, phi057 or phi112, which are sequences located within the Opaque-2 (O2) gene; the endosperm phenotypes were observed visually. In the BC(1)F(1) populations, the frequency of heterofertilization was 0 approximately 4.545%. This result indicates that there may be up to a 5% difference between the selections based on endosperm phenotype and plant genotype in a backcross breeding program. Molecular marker assisted selection should be a more accurate selection procedure. In addition, we discuss the relationship between the endosperm heterofertilization of o2 maize and the variations within the O2 locus.