Moderate loss of cerebellar purkinje cells after chronic bilateral common carotid artery occlusion in rats
Moderate loss of cerebellar purkinje cells after chronic bilateral common carotid artery occlusion in rats
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DOI：
10.1007/s00401-007-0204-y
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发表时间：
2007-05-01
影响因子：
12.7
通讯作者：
Schmidt-Kastner, Rainald
中科院分区：
文献类型：
--
作者：
Kantor, Orsolya;Schmitz, Christoph;Schmidt-Kastner, Rainald
中度缺血（少血、低灌注）的病理效应与痴呆中的血管因子相关。成年Wistar大鼠慢性双侧颈总动脉闭塞（BCCAO）可诱导缺血，并导致基因表达的急性变化、皮质星形胶质细胞的亚急性变化和白色物质束的长期变化，同时在很大程度上保留了前脑区的神经元。基底动脉的扩张和重塑确保了前脑的血流。本研究采用传统的神经病理学分析、免疫组化和高精度设计为基础的体视学方法，研究了BCCAO 6个月后小脑中的缺氧敏感性浦肯野细胞。小脑蚓部浦肯野细胞核形态异常。体视学分析显示，BCCAO动物的小脑蚓部浦肯野细胞平均总数在统计学上显著小于对照动物（d = 11.8%; P <0.0001）。BCCAO对小脑蚓部或整个小脑的分子层、颗粒细胞层和白色物质的平均体积无显著影响。基底动脉的重构表明，继发性血管扰动可能是BCCAO对小脑浦肯野细胞的影响。
Pathological effects of moderate ischemia (oligemia, hypoperfusion) are relevant in relation to vascular factors in dementia. Chronic bilateral common carotid artery occlusion (BCCAO) in adult Wistar rats induces oligemia and leads to acute changes in gene expression, subacute changes in cortical astrocytes and prolonged changes in white matter tracts, while largely sparing neurons in the forebrain areas. Dilation and remodeling of the basilar artery ensures blood flow to the forebrain. The present study examined the hypoxia-sensitive Purkinje cells in the cerebellum after 6 months of BCCAO using conventional neuropathological analysis, immunohistochemistry and high-precision design-based stereologic methods. Purkinje cells in the vermis region revealed abnormally shaped nuclei. A stereologic analysis showed that the mean total number of Purkinje cells within the vermis was statistically significantly smaller in the BCCAO animals than in the control animals (d = 11.8%; P < 0.0001). BCCAO had no significant effect on the mean volumes of the molecular layer, granule cell layer and white matter in the vermis or the entire cerebellum. Remodeling of the basilar artery indicated that secondary vascular perturbations might be responsible for the effects of BCCAO on the cerebellar Purkinje cells.