Osteoconductive composite graft based on bacterial synthesized hydroxyapatite nanoparticles doped with different ions: From synthesis to in vivo studies
Osteoconductive composite graft based on bacterial synthesized hydroxyapatite nanoparticles doped with different ions: From synthesis to in vivo studies
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DOI：
10.1016/j.nano.2016.01.020
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发表时间：
2016-07-01
影响因子：
5.4
通讯作者：
Mostaghaci, Babak
中科院分区：
文献类型：
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作者：
Ahmadzadeh, Elham;Talebnia, Farid;Mostaghaci, Babak
为了修复受损的骨组织，需要骨导电性骨移植替代物来增强成骨细胞的再生潜能。纳米羟基磷灰石是一种用于骨组织工程的生物活性陶瓷。本研究以产氧肠杆菌为原料，采用生物矿化方法合成了碳酸羟基磷灰石（cHA）和锌镁取代羟基磷灰石（Zn-Mg-HA）纳米颗粒。采用合适的粉末表征方法对样品的结构相组成和形貌进行了分析。然后，用聚乙烯醇和cHA、Zn-Mg-HA样品制备了复合接枝。然后在兔胫骨截骨模型中研究移植物的体内成骨潜能。组织学、影像学和形态学研究表明，移植4周后移植物被新形成的骨组织矿化，无炎症或感染迹象。这些组织形态测量结果表明，这种人造移植物可以作为一种有效的骨导电性骨组织替代品。(C) 2016 Elsevier Inc.版权所有。
To repair damaged bone tissues, osteoconductive bone graft substitutes are required for enhancement of the regenerative potential of osteoblast cells. Nanostructured hydroxyapatite is a bioactive ceramic used for bone tissue engineering purposes. In this study, carbonate hydroxyapatite (cHA) and zinc-magnesium substituted hydroxyapatite (Zn-Mg-HA) nanoparticles were synthesized via biomineralization method using Enterobacter aerogenes. The structural phase composition and the morphology of the samples were analyzed using appropriate powder characterization methods. Next, a composite graft was fabricated by using polyvinyl alcohol and both cHA and Zn-Mg-HA samples. In vivo osteogenic potential of the graft was then investigated in a rabbit tibial osteotomy model. Histological, radiological and morphological studies showed that the graft was mineralized by the newly formed bone tissue without signs of inflammation or infection after 4 weeks of implantation. These histomorphometric results suggest that the fabricated graft can function as a potent osteoconductive bone tissue substitute. (C) 2016 Elsevier Inc. All rights reserved.