Wide distribution of Plasmodium ovale in Myanmar
Wide distribution of Plasmodium ovale in Myanmar
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DOI：
10.1046/j.1365-3156.2002.00857.x
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发表时间：
2002-03-01
影响因子：
3.3
通讯作者：
Kawamoto, F
中科院分区：
文献类型：
--
作者：
Win, TT;Lin, K;Kawamoto, F
缅甸以前从未报告过卵形疟原虫的存在。使用1996年至2000年期间在全国许多村庄获得的血液样本，用种特异性引物进行半巢式或全巢式聚合酶链反应（PCR），然后用琼脂糖凝胶电泳检测扩增产物，进行疟原虫种的分子诊断。卵形疟原虫的存在也通过另一种基于PCR的诊断得到证实，即使用种特异性探针的微量滴定板杂交（MPH）方法。这两种方法都是针对四种人疟原虫核糖体小亚基RNA基因的A型。在1323例PCR阳性患者中，65例（4.9%）的样本中扩增出卵形疟原虫DNA，巢式PCR和MPH获得的结果完全一致。只有4例卵圆疟原虫感染患者为单一种属感染，其他患者均为恶性疟原虫、间日疟原虫和/或三日疟原虫合并感染。在6名受试者中观察到四重感染。通过常规Giemsa染色薄涂片显微镜或吖啶橙染色薄涂片荧光显微镜检查，仅在19例患者中发现具有典型卵形疟原虫形态的寄生虫。在缅甸南部、中部和西部地区的村庄发现了卵形疟原虫感染，表明卵形疟原虫可能在该国广泛分布。
The presence of Plasmodium ovale has never been previously reported in Myanmar. Using blood samples obtained in many villages across the country between 1996 and 2000, molecular diagnosis of Plasmodium species was made with semi- or full-nested polymerase chain reaction (PCR) with species-specific primers, followed by agarose gel electrophoresis to detect amplification products. The presence of P. ovale was also confirmed with another PCR-based diagnosis, the microtiterplate hybridization (MPH) method using species-specific probes. Both methods target the A type of the small subunit ribosomal RNA gene of the four human malaria parasites. Plasmodium ovale DNA was amplified in samples from 65 (4.9%) of 1323 PCR-positive patients, with perfect agreement between results obtained by nested PCR and MPH. Only four P. ovale-infected patients had single-species infection; all others were coinfected with P. falciparum, P. vivax and/or P. malariae. Quadruple infections were observed in six subjects. Parasites with typical P. ovale morphology were found in only 19 patients by conventional microscopy of Giemsa-stained thin smears or fluorescence microscopy of acridine orange-stained thin smears. Plasmodium ovale infections were found in villages situated in the southern, central and western regions of Myanmar, suggesting that P. ovale may be widely distributed in this country.