Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release
Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release
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DOI：
10.1073/pnas.93.10.4760
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发表时间：
1996-05-14
影响因子：
11.1
通讯作者：
Sudhof, TC
中科院分区：
文献类型：
--
作者：
McMahon, HT;Bolshakov, VY;Sudhof, TC
突触素（syp I）是一种突触囊泡膜蛋白，约占总囊泡蛋白的7%。多种证据表明syp I参与了许多神经末梢功能。为了测试这些，我们已经破坏了小鼠syp I基因，缺乏syp I的突变小鼠是可行的和可生育的。除了小突触泡蛋白/VAMP II减少外，未观察到突变体大脑结构和蛋白质组成的变化。突触传递正常，突触可塑性或释放概率没有可检测到的变化。我们的数据表明，一个主要的突触囊泡膜蛋白是不是突触传递必不可少的，这表明它的功能是多余的，或者它有一个更微妙的功能不明显的测定。
Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximate to 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a number of nerve terminal functions. To test these, we have disrupted the murine syp I gene, Mutant mice lacking syp I were viable and fertile. No changes in the structure and protein composition of the mutant brains were observed except for a decrease in synaptobrevin/VAMP II. Synaptic transmission was normal with no detectable changes in synaptic plasticity or the probability of release. Our data demonstrate that one of the major synaptic vesicle membrane proteins is not essential for synaptic transmission, suggesting that its function is either redundant or that it has a more subtle function not apparent in the assays used.