Disruption of the murine PIASx gene results in reduced testis weight
Disruption of the murine PIASx gene results in reduced testis weight
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DOI：
10.1677/jme.1.01666
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发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Palvimo, JJ
中科院分区：
文献类型：
--
作者：
Santti, H;Mikkonen, L;Palvimo, JJ
PIASx属于皮亚斯蛋白家族，其成员调节几种转录因子的活性并在类小泛素化途径中充当E3连接酶。PIASx基因在睾丸中高度表达，表明在精子发生中的作用。为了研究PIASx在体内的功能，我们破坏了小鼠中的PIASx基因。有趣的是，基因敲除小鼠是可行的和可生育的。尽管生育力正常，但突变动物的睾丸重量减少，睾丸细胞凋亡数量增加。此外，突变小鼠的精子数量趋于减少，但其精子细胞的质量是正常的。在LH和FSH的血清水平或睾丸内睾酮浓度之间的基因敲除动物和野生型同窝仔没有观察到显着变化。在敲除小鼠中未观察到其他皮亚斯蛋白mRNA的补偿性增加。这些结果表明，PIASx是需要定量，而不是定性的正常精子发生。
PIASx belongs to the PIAS protein family, the members of which modulate activities of several transcription factors and act as E3 ligases in the sumoylation pathway. The PIASx gene is highly expressed in testis, suggesting a role in spermatogenesis. To investigate the function of PIASx in vivo, we have disrupted the PIASx gene in mice. Interestingly, the knockout mice were viable and fertile. Despite the normal fertility, the testis weight of the mutant animals was reduced and their number of apoptotic testicular cells was increased. Also, the sperm count of mutant mice tended to be reduced, but the quality of their sperm cells was normal. No significant changes were observed in the serum levels of LH and FSH or in the intratesticular testosterone concentration between the knockout animals and their wild-type littermates. Compensatory increases in other PIAS protein mRNAs were not observed in the knockout mice. These results imply that PIASx is required quantitatively rather than qualitatively for normal spermatogenesis.